April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Simultaneous Indocyanine Green and Fluorescein Angiography of the Iris Using Confocal Scanning Laser Ophthalmoscope in the Albino Rabbit
Author Affiliations & Notes
  • J. Wu
    Tianjin Medical University Eye Center, Tianjin, China
  • L. Su
    Tianjin Medical University Eye Center, Tianjin, China
  • S. Lin
    Tianjin Medical University Eye Center, Tianjin, China
  • Y. Zhou
    Tianjin Medical University Eye Center, Tianjin, China
  • G. Sun
    Tianjin Medical University Eye Center, Tianjin, China
  • X. Zhou
    Tianjin Medical University Eye Center, Tianjin, China
  • X. Li
    Tianjin Medical University Eye Center, Tianjin, China
  • Footnotes
    Commercial Relationships  J. Wu, None; L. Su, None; S. Lin, None; Y. Zhou, None; G. Sun, None; X. Zhou, None; X. Li, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5786. doi:
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      J. Wu, L. Su, S. Lin, Y. Zhou, G. Sun, X. Zhou, X. Li; Simultaneous Indocyanine Green and Fluorescein Angiography of the Iris Using Confocal Scanning Laser Ophthalmoscope in the Albino Rabbit. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5786.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To study iris angiography (IA) in the albino rabbit using the mixture of Indocyanine green (ICG) and fluorescein (FA).

Methods: : Digital Iris angiograms were performed by using confocal scanning laser ophthalmoscope (Heidelberg HRAI) in 6 albino rabbits. One eye of each rabbit was randomly used. We used a modified location-assisted laser devices to ensure that the scanning mirror was always perpendicular to the surface of the iris. Photographs were taken at 0.5 sec interval until the full arteriovenous phase, then at 10 sec intervals until 5 min, late photographes were taken at 10, 15, and 20 min after injection of the mixture of ICG and FA. IAs were performed in 6 rabbits once a week with different doses, the dose at 1st week was 0.2 ml 10% FA and 2 mg ICG, the 2nd week was 0.5 ml 10% FA and 5 mg ICG, the 3rd week was 0.8 ml 10% FA and 8 mg ICG. 3 sets of images of the same rabbit were compared for the consistency of iris vascular morphology and area with image processing software.

Results: : Digital angiography showed that dynamic full process of iris vessels in the albino rabbit. The emergence time(ET)of FA was 5~8 sec, average 6.4 sec, ET of ICG was 6~10 sec, average 8.1 sec. Firstly, dye appeared in the major arterial circle formed by 4 large branches, two entering temporally and two nasally, and then radial arterial branches from the major circle arrived at the pupil boundary. No evidence of the minor arterial circle was noted. In near pupil marginal zone, capillaries drained into the radial venous channels towards the limbus. Different doses of mixture lead to different maintenance time. In 0.2 ml 10% FA and 2 mg ICG, FA extinguished rapidly in 20 sec while ICG maintained 2 min, no obvious FA or ICG leakage were seen in chamber. In 0.5 ml 10% FA and 5 mg ICG, normal blood vessels can also be clearly displayed by ICG in 4 min and no leakage, but in FA clear vessels maintained about 30 sec and the structure of the iris became fuzzy due to quick leakage. In 0.8 ml 10% FA and 8 mg ICG, in ICG vascular patterns were showed in up to 8 min while in FA only 20 sec with subsequently significant leakage appeared as streams on the surface of the iris until the dye disappeared from the iris vessels, the diffuse and intense aqueous fluid lasted 1 d. The consistency of the digital image of vascular area of IA in the same rabbit can be proved among different doses by using image processing software.

Keywords: iris • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 
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