April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
The Role of Retinal Müller Cell-Produced VEGF in Ischemia Induced Vascular Leakage
Author Affiliations & Notes
  • Y. Bai
    Department of Medicine, Soochow University, Suzhou, China
    Department of Medicine and Harold Hamm Oklahoma Diabetes Center, the University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  • J.-X. Ma
    Department of Medicine and Harold Hamm Oklahoma Diabetes Center, the University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  • Y.-Z. Le
    Department of Medicine/Cell Biology and Harold Hamm Oklahoma Diabetes Center, the University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
    Dean A. McGee Eye Institute, Oklahoma city, Oklahoma
  • Footnotes
    Commercial Relationships  Y. Bai, None; J.-X. Ma, None; Y.-Z. Le, Reagent from Genentech Inc., R.
  • Footnotes
    Support  NIH grants RR17703 and EY12190, ADA grant 1-06-RA-76, AHAF grant M2008-059, FFB grant BR-CMM-0808-0453-UOK and unrestricted grants from Hope for Visiion and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5899. doi:
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    • Get Citation

      Y. Bai, J.-X. Ma, Y.-Z. Le; The Role of Retinal Müller Cell-Produced VEGF in Ischemia Induced Vascular Leakage. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5899.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Recent studies have shown that the overexpression of vascular endothelial growth factor (VEGF) is crucial for retinal neovascularization and vascular leakage, major pathological changes in diabetic retinopathy (DR). Retinal Müller cell is a major VEGF producer in the retina and is thought to be responsible for pathological changes in DR. To investigate the significance of Müller cell-produced VEGF in DR, we generated mice that had VEGF disruption in retinal Müller cells. The goal of this study is to determine the role of Müller cell-produced VEGF in ischemia-induced vascular leakage and its underline mechanism.

Methods: : The conditional VEGF knockout mice were subjected to hypoxia using an oxygen-induced retinopathy (OIR) model. Retinal vascular leakage in the retina was determined by measurement of albumin leakage in the retina using immunohistochemistry (IHC) and Western-blot. Expression of occludin, a tight junction protein, in the retinal vessels was determined by IHC and Western-blot.

Results: : Loss of Müller cell-produced VEGF caused a reduction in ischemia-induced vascular leakage and prevented the decrease of occludin expression.

Conclusions: : Our study suggests that the Müller cell-produced VEGF plays a crucial role in ischemia-induced vascular leakage through attenuation of tight-junction protein expression in retinal vessels.

Keywords: Muller cells • vascular endothelial growth factor • diabetic retinopathy 
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