Purchase this article with an account.
L. Kowalczuk, E. Touchard, C. Goumeaux, L. Jonet, P. Bigey, J.-C. Jeanny, F. Behar-Cohen; Sustained Intraocular Production of Placental Growth Factor (pgf), Using Ciliary Muscle Electrotransfer: A Model for Early Diabetic Retinopathy Changes?. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5906.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The purpose of this study was to evaluate clinical and histopathological ocular changes induced by sustained PGF over-expression in rat eyes using electrotransfer (ET).
Ciliary muscle injection of pVAX2-rPGF (30µg) combined with ET (200V/cm) was performed in pigmented and non-pigmented rat eyes. Control eyes received ciliary muscle ET of saline. Clinical signs induced by PGF secretion in ocular media were followed up until three months after ET by slit lamp examination and by fluorescein angiography. Ciliary bodies were dissected and used for the evaluation of the PGF mRNA levels. The effect of PGF secretion on ocular structures was evaluated by semi-thin sections analysis and by immunohistochemistry for PGF, GFAP, alpha-SMA, Von Willbrand. Retinal vasculature was analyzed by lectin and GFAP staining on flat-mounted retina.
Compared with control eyes, PGF expression was significantly increased in the ciliary muscle eyes receiving pVAX2-rPGF ET as confirmed by PCR and immunohistochemitry. Rat PGF secretion induced retinal pigmented epithelial barrier breakdown with subsequent retinal edema, together with retinal vessel abnormalities (diffusion, diffusing microaneurysms, irregularities...). Histological analysis showed that associated to the retinal vessel abnormalities, pre retinal GFAP positive membranes could be observed.
Over expression of PGF induces retinal abnormalities resembling to diabetic retinopathy. These observations confirm that PGF/ VEGFR1 may be potential targets for the treatment of diabetic retinopathy.
This PDF is available to Subscribers Only