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J. E. Mancini, J. C. Basabe, J. O. Croxatto, J. E. Gallo; Proliferative Diabetic Retinopathy Changes in a Female Rat Model of Type 2 Diabetes Fed on High Fat Rich Diet. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5910.
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To evaluate long-term retinal changes in diabetic rats.
Type 2 diabetes model was constituted by a group of 10 female Wistar neonatal rats. They received an intraperitoneal injection 45 mg/kg streptozotocin (SZT) at day 2 of life. Rats were fed by a high-fat-diet (HFD) from week 8 onwards and killed after 2 years of diabetes. A control group was made up of non-diabetic rats. Transverse retinal sections were analyzed by immunohistochemistry using glial fibrillary acidic protein (GFAP) and the receptor-of-advanced glication-end-product (RAGE), and by histology using PAS and HE.
Proliferative retinopathy was only found in diabetic animals. This group also showed rubeosis iridis-like changes. Immunostaining of GFAP and RAGE was similar in diabetic and controls.
This model of diabetes reproduces late stages of diabetic retinopathy and associated complications. It could be useful for better understanding the role of HFD in retinopathy development.
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