April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Immuno-Modulatory Effect of Human Fetal Retinal Pigment Epithelial (fRPE) Cells on Monocyte Differentiation and Maturation
Author Affiliations & Notes
  • S. Chakrabarty
    Laboratory of Immunology,
    NEI, NIH, Bethesda, Maryland
  • Z. Li
    Laboratory of Immunology,
    NEI, NIH, Bethesda, Maryland
  • A. Maminishkis
    Section on Epithelial and Retinal Physiology and Disease,
    NEI, NIH, Bethesda, Maryland
  • B. Liu
    Laboratory of Immunology,
    NEI, NIH, Bethesda, Maryland
  • S. Miller
    Section on Epithelial and Retinal Physiology and Disease,
    NEI, NIH, Bethesda, Maryland
  • R. B. Nussenblatt
    Laboratory of Immunology,
    NEI, NIH, Bethesda, Maryland
  • Footnotes
    Commercial Relationships  S. Chakrabarty, None; Z. Li, None; A. Maminishkis, None; B. Liu, None; S. Miller, None; R.B. Nussenblatt, None.
  • Footnotes
    Support  Intramural research program of the National Eye Institute of NIH.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 5921. doi:
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      S. Chakrabarty, Z. Li, A. Maminishkis, B. Liu, S. Miller, R. B. Nussenblatt; Immuno-Modulatory Effect of Human Fetal Retinal Pigment Epithelial (fRPE) Cells on Monocyte Differentiation and Maturation. Invest. Ophthalmol. Vis. Sci. 2009;50(13):5921.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Ocular immune privileged tissue is well known. Human fRPE cells have been shown to inhibit T cell activation, but little is known about their effect on monocytes and macrophages. We evaluated the contribution of fRPE cells, on monocytes and macrophages, major mediators of inflammation.

Methods: : CD14+ monocytes were co-cultured with human primary fetal RPE cells in the presence of M-CSF for 3 or 7 days. Phenotyping for surface markers and cytokine profiles were analyzed to assess the differentiation of monocytes into macrophages. As a control, monocyte were also cultured alone or co-cultured with fibroblasts in the presence of M-CSF. To evaluate, the effect of fRPE cells on maturation of macrophages, CD14+ monocytes were differentiated to maturation by treating with M-CSF for 6 days and then co-cultured with fRPE cells with or with out LPS stimulation and was analyzed by macrophage surface markers, cytokine profiles and phagocytosis.

Results: : Monocytes driven by M-CSF differentiated into macrophages by increasing their cellular size and expressed high levels of CD163, CXCR4, DR and CD25. However, all those markers were down-regulated by the presence of fRPE cells. Fibroblasts from the same donors did not have this effect. This effect was noted at a late stage of differentiation. Macrophages differentiated in the presence of fRPE cells produced more IL-10 than those without. Meanwhile, fRPE cells up-regulated CD163 and CXCR4 expression but down-regulated DR expression as well as phagocytosis during the maturation of macrophages.

Keywords: retinal pigment epithelium • immunomodulation/immunoregulation • phagocytosis and killing 
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