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P. J. Accola, P. A. Moore, K. P. Carmichael, J. D. Lauderdale; Evaluation of Corneal Healing, Limbal Progenitor Cells, and Vascularization in Wounded Pax 6+/+ and Pax 6+/- Mice. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6302.
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Describe ocular pathology and compare corneal wound healing in Wild Type (WT) (Pax6+/+) and Small eye (Sey) (Pax 6+/-) mice.
WT (n=84) and Sey (n=40) mice (age 2-3 months) were examined using biomicroscopy. Corneal wounding was performed by applying an n-heptanol saturated disk to OS for 60 seconds. Mice were evaluated on days 1, 2, 3 (if fluorescein positive on day 2), 4, 7, 14, and 28 post wounding. Each day, 3-5 mice were euthanized. Eyes were enucleated and placed in 4% paraformaldehyde. Immunohistochemistry (p63, sVEGFR-1) was performed. Days to negative fluorescein staining were compared using the Chi-Square test. The number of p63 staining basal cells was recorded and statistically evaluated by ANOVA. Staining for sVEGFR1 was recorded as positive or negative.
Anterior segments in all WT mice were normal. Pathology in Sey mice included corneal opacity (n=35), corneal vascularization (n=20), iris hypoplasia (n=36), and anterior cortical cataract (n=27). Application of n-heptanol resulted in removal of corneal epithelium in all mice. There was no significant difference in the amount of cornea wounded in WT and Sey mice (p>0.05). There was a statistically significant delay in corneal wound healing in Sey mice at days 2 and 3 when compared to WT mice (p<0.05). There was no significant difference in p63 staining (p>0.05). All mice exhibited comparable sVEGFR1 staining.
The corneal healing delay in Sey mice does not appear to be due to a deficiency in p63 cellular expression. The comparable expression of sVEGFR1 suggests that it alone is likely not responsible for the corneal vascularization present in Sey mice.
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