Purchase this article with an account.
I. Alcalde, S. del Olmo-Aguado, P. Gallego, P. Perez-Merino, R. Cantalapiedra, L. Ibares-Frias, J. Merayo-Lloves, T. Blanco; Influence of ErbB 2 Receptor on the Innervation of Corneal Stroma During a Wound Healing Process. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6303.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To characterize a neural-like phenotype of stromal cells following corneal injury and to stablish the role of ErbB 2 receptor during the reinnervation process of the cornea.
PRK operated mice serve as model of corneal injury and wound healing process. Whole mounted corneas were incubated with antibodies against Neurofilament 200, GFAP, S100, NCAM, SMA and ErbB 2 receptor, in order to demonstrate the presence of these markers in the newly formed stromal tissue. Western Blot analysis were performed to evaluate the evolution of the expression of these markers.
After corneal injury, numerous cell processes expresses strongly the ErbB 2 receptor in the stroma. These cells accumulate preferently at the subepithelial layers. This profiles are closely related to axonal processes corresponding to regenerating axons and present a Schwann cell-like biochemical phenotype, as demonstrated by their immunoreactivity against GFAP and S100 protein.
Axons could exert a potent effect over stromal cells and change temporarily their phenotype to improve reinnervation. This effect is mediated via ErbB 2 receptor activity.
This PDF is available to Subscribers Only