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G. Hernandez-Martinez, J. Morales-Martínez, C. Santacruz-Valdés, M. Linares, M. C. Jiménez-Martínez; Changes on the Expression of Cell Surface Markers Associated with Central/Peripheral Memory on CD57+ T Cells After Antigenic Specific Stimulation With Der p in Allergic Conjunctivitis Patients. Invest. Ophthalmol. Vis. Sci. 2009;50(13):6332.
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© ARVO (1962-2015); The Authors (2016-present)
To determine CD57+ expression on T cells stimulated with Dermatophagoides pteronyssinus (Der p) and to determine cell surface markers associated with central or peripheral memory on cells from conjunctivitis patients.
Mononuclear cells were obtained from peripheral blood to determine CD57, CD27, CD45RA, CD45RO, CCR7 and Ki-67 expression on cells before and after antigenic stimulation with Der p. Data were analyzed by flow cytometry. Mann-Whitney test was used to compare statistical differences. Significant correlation was performed with Pearson test. Significant differences were considered when p<0.05.
We observed increased frequency of CD57 cells before (p=0.0001) and after (p=0.0003) Der p stimulation in conjunctivitis patients. CD45 isoforms index (CD45RA/CD45RO) was 3.3:1 before stimulation (p=0.02) and after stimulation was 3.1:1 (p=0.003). Positive correlations were observed when CD45RA was associated with CCR7 and CD27 (r=1; p<0.0001), negative correlations were observed when CD45RO was associated with CCR7 and CD27 (r=-1; p<0.0001).
Frequency of CD57 T cells increase after antigenic specific stimulation. We observed two cells subsets in conjunctivitis patients: one of them was CD27+CCR7+CD45RA+ (central memory), the other one was CD27-CCR7-CD45RO+ (peripheral memory). Functional studies are needed to elucidate the real role of these two subsets in patients with allergic conjunctivitis.Acknowledgments: CONACYT 71291
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