Purchase this article with an account.
H.-W. Kwak, M.-S. Kim, S.-W. Moon, S.-Y. Yu; Comparison of Vitreolytic Effect in Rabbit Eyes: Plasmin, Hyaluronidase, and Their Mixtures. Invest. Ophthalmol. Vis. Sci. 2007;48(13):102.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To quantify and compare the vitreolytic effect of plasmin, hyaluronidase, and their mixtures.
Thirty-six rabbits were randomized into three groups: (A) Twelve rabbits, intravitreal injection of plasmin 1 U + hyaluronidase 10 U/0.1 ml into right eye; (B) Twelve rabbits, plasmin alone(1 U/0.1 ml); (C) Twelve rabbits, hyaluronidase alone(10 U/0.1 ml). The left eye of each rabbit was used as control which was injected phosphate buffered saline(PBS) 0.1 ml. The eyes were enucleated 1 hour and 24 hours after injection. Enucleated eyeballs were frozen rapidly and then melted in room temperature. Volume of fluid-vitreous and gel-vitreous were measured with micropipette using the difference of melting point. Statistical analysis was performed.
Remained gel-vitreous volume were measured 52.53%, 60.26%, 59.18%, and 76.46% of group A, B, C and control group after 1 hour enucleation. In cases of enucleated eyes after 24 hours, remained gel-vitreous volume were measured 44.55%, 56.65%, 56.05%, and 74.65%, respectively. Group A, B, and C showed statistically significant difference against the control group. Group A (plasmin + hyaluronidase) represented less remained gel-vitreous volume than plasmin or hyaluronidase single injection at the same time.
Intravitreal injection of plasmin + hyaluronidase showed more vitreolytic effect than plasmin or hyaluronidase single injection at the same time.
This PDF is available to Subscribers Only