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T. Hayashi, S. Yamagami, K. Tanaka, S. Yokoo, T. Usui, S. Amano, N. Mizuki; Characterization of Immune Response in Mouse Corneal Endothelial Cell Transplantation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):186.
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Corneal endothelial cell (CEC) transplantation should be applied for clinical use in the near future. We established a mouse bullous keratopathy model and investigated the immune response in CEC transplantation.
The anterior chamber of the eyes in recipient Balb/c mice were treated with 0.05% benzalkonium chloride and corneas with bullous keratopathy were created. Donor corneas were divided into 4 groups of (1)normal Balb/c full-thickness corneas (group with an isograft of a full-thickness cornea), (2)Balb/c corneas denuded of endothelium (group with a corneal isograft without endothelium), (3)allograft of full-thickness corneas from normal C3H/He mice (minor and major histocompatibility complex-incompatible) (group with an allograft of a full-thickness cornea), and (4)reconstructed corneas with immortalized C3H/He mouse corneal endothelial cell grafted to Balb/c corneas denuded of endothelium (group with a corneal allograft with endothelium), and full-thickness corneal transplantation was performed. The corneal grafts were defined as rejected immunologically when they became opaque and the iris vessels could not be recognized clearly. Delayed type hypersensitivity (DTH) response was tested in the fourth week postoperatively.
The group with an isograft of full-thickness corneas retained corneal transparency in all cases, whereas none of the corneas recovered transparency in the group with a corneal isograft without endothelium until 6 weeks after surgery. The rate of rejection in the group with an endothelial graft was significantly lower than in the group with an allograft of full-thickness corneas (p<0.008). DTH response was detected in the group with an allograft of a full-thickness cornea but not in the group with an endothelial graft.
In a mouse bullous keratopathy model, CEC transplantation shows lower rate of immune response than full-thickness corneal transplantation.
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