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T. Kyomoto, H. Imai, N. Senda, T. Yanagidaira, S. Arai-gaun, N. Katai; ßB2-Crystallin Regulated the Ganglion Cell Death After Ischemia-Reperfusion Injury. Invest. Ophthalmol. Vis. Sci. 2007;48(13):239.
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© ARVO (1962-2015); The Authors (2016-present)
We presented that ßB2-crystallin protein and mRNA were upregulated in rat retinal ischemia-reperfusion injury and the peak time point of these expression was at 12 hours after reperfusion in ARVO, 2006. To investigate the roles ßB2-crystallin played in rat retinal ischemia-reperfusion injury by using the ßB2-crystallin short interfering RNA (siRNA).
Retinal ischemia for 60 minutes was induced in rats by increasing the intraocular pressure to 110 mm Hg. To inhibit the upregulation of ßB2-crystallin, intravitreal injection of ßB2-crystallin siRNA was performed before ischemia. The expression of ßB2-crystallin was studied on Western blotting and immunohistochemical methods.
On Western blotting, ßB2-crystallin was downregulated at 12 hours after reperfusion. On immunohistochemical methods, the number of cells in the ganglion cell layer changed at 24 hours after ischemia-reperfusion injury.
ßB2-crystallin may regulate the retinal ganglion cell death after ischemia-reperfusion injury.
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