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J. J. Steinle, L. S. McFadyen-Ketchum, V. C. Chin, F. A. Capoccia, Jr.; Increased Expression of Apoptotic Markers in Choroid-RPE Complex in Normal Aging. Invest. Ophthalmol. Vis. Sci. 2007;48(13):27.
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To characterize changes in steady-state mRNA expression and protein levels of apoptotic markers in the choroid-RPE complex during normal aging of the rat.
Male BN x Fisher 344 F1 hybrids were used for these studies at 8, 22, and 32 months of age. At the appropriate age, choroid-RPE complex was dissected from the eyecup for real-time PCR and western blot analyses. Gene expression and protein levels of p53, Fas, Fas ligand, Bax, Bcl-2, and caspase 3 were for analyses.
With increasing age, there was a significant increase in both gene expression and protein levels of Fas ligand, while only gene expression of Fas was increased with age. Protein levels of Bax were increased with age, with no changes noted in Bcl-2 expression. While gene expression for p53 was increased in older animals, protein expression was decreased. There was a trend for increased caspase 3 levels with increasing age, suggesting that apoptosis is favored with increasing age in this tissue.
Normal aging results in an upregulation of gene expression and protein levels of some apoptotic markers in the choroid-RPE complex. Further studies are designed to determine the cellular pathways involved and cellular localization of these markers.
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