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Y.-P. Tsao; Pigment Epithelium-Derived Factor Induces THP-1 Macrophage Apoptosis and Necrosis by the Induction of the Peroxisome Proliferator-Activated Receptor Gamma. Invest. Ophthalmol. Vis. Sci. 2007;48(13):545.
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Recently, PEDF has also been suggested to have an anti-inflammatory effect, although the mechanisms have not been fully elucidated.
Aopptosis was assayed by TUNEL assay, cytochrome c release, p53 overexpression and cleavage of caspase-9, and caspase-3
PEDF dose- and time-dependently induces both apoptosis and necrosis of human THP-1 monocytic leukemia cell line-derived macrophage cells. The apoptosis and necrosis were significantly decreased when cells were pretreated with either peroxisome proliferator-activated receptor gamma (PPARγ) small interfering RNA or PPARγ inhibitor GW9662.
Our observations indicate that PEDF induces THP-1 macrophage apoptosis and necrosis through the induction of PPARγ overexpression. This suggests a novel mechanism through which PEDF can modulate inflammation.
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