May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Pigment Epithelium-Derived Factor Induces THP-1 Macrophage Apoptosis and Necrosis by the Induction of the Peroxisome Proliferator-Activated Receptor Gamma
Author Affiliations & Notes
  • Y.-P. Tsao
    MacKay Memorial Hospital, Taipei, Taiwan
  • Footnotes
    Commercial Relationships Y. Tsao, None.
  • Footnotes
    Support MMH 94006
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 545. doi:
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    • Get Citation

      Y.-P. Tsao; Pigment Epithelium-Derived Factor Induces THP-1 Macrophage Apoptosis and Necrosis by the Induction of the Peroxisome Proliferator-Activated Receptor Gamma. Invest. Ophthalmol. Vis. Sci. 2007;48(13):545.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Recently, PEDF has also been suggested to have an anti-inflammatory effect, although the mechanisms have not been fully elucidated.

Methods:: Aopptosis was assayed by TUNEL assay, cytochrome c release, p53 overexpression and cleavage of caspase-9, and caspase-3

Results:: PEDF dose- and time-dependently induces both apoptosis and necrosis of human THP-1 monocytic leukemia cell line-derived macrophage cells. The apoptosis and necrosis were significantly decreased when cells were pretreated with either peroxisome proliferator-activated receptor gamma (PPARγ) small interfering RNA or PPARγ inhibitor GW9662.

Conclusions:: Our observations indicate that PEDF induces THP-1 macrophage apoptosis and necrosis through the induction of PPARγ overexpression. This suggests a novel mechanism through which PEDF can modulate inflammation.

Keywords: apoptosis/cell death • inflammation • cytokines/chemokines 
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