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S. Mohand Said, Sr., Y. Yang, M. Simonutti, V. Fontaine, T. Léveillard, J. A. Sahel; Reactive Protection of Retinal Function Related to Transplantation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):622.
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© ARVO (1962-2015); The Authors (2016-present)
Our aim is to demonstrate that transplantation of neural retina or selected photoreceptor not only limits death of cones, but also prevents loss of visual function by using a retinal degeneration model-P23H rat.
Heterozygote P23H rats (3months) were transplanted subretinally in one eye with retinal sheets (2mm x 4mm) obtained from postnatal 8 day Sprague Drawley rats. After 6 months, transplanted retinas were analysed by immunocytochemistry and histology. We quantified the numbers of retinal cones, using a stereological approach to obtain unbiased samples after immunolabeling with peanut agglutinin (PNA) lectin to label cones and anti-opsin Rho-4D2 antibody to label rods. ERG recording was assessed for the analysis of visual function.
Histology and ERG show photoreceptor degeneration of P23H rats following stages, specially rapid from 2 to 4 months. During this period, scotopic B wave amplitude decreases faster than photopic B wave amplitude. By transplantation with neural retina (14 rats) or photoreceptor (18 rats), remaining cones were average of 9.2% (P<0.01) and 9.9% (P<0.01) more than that of the control. Both types of transplantation had dramatic effects on photopic ERG (B wave amplitude, P<0.05). Increase of cone number corresponds to rising of photopic ERG B wave amplitude.
Our study provides a better understanding of photoreceptor survival and degeneration pathways, both of cell quantity and cell function. Furthermore, this fact confirms effective application of retinal sheet or photorecptor transplantation in Retinitis Pigmentosa.
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