May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Heparan Sulfate Is Required for the Development of Optic Nerve Head
Author Affiliations & Notes
  • M. Ogata
    Department of Opthalmology and Visual Sciences, Kumamoto University, Kumamoto City, Japan
  • M. Inatani
    Department of Opthalmology and Visual Sciences, Kumamoto University, Kumamoto City, Japan
  • K. Iwao
    Department of Opthalmology and Visual Sciences, Kumamoto University, Kumamoto City, Japan
  • H. Tanihara
    Department of Opthalmology and Visual Sciences, Kumamoto University, Kumamoto City, Japan
  • Footnotes
    Commercial Relationships M. Ogata, None; M. Inatani, None; K. Iwao, None; H. Tanihara, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1229. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Ogata, M. Inatani, K. Iwao, H. Tanihara; Heparan Sulfate Is Required for the Development of Optic Nerve Head. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1229.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: Heparan sulfate (HS) and HS proteoglycan are known to bind to numerous proteins such as adhesion molecules and chemoattractants for optic nerve guidance. In the present study, we investigated whether the morphogenesis of optic nerve head depends on HS.

Methods:: We conditionally disrupted a HS-polymerizing enzyme, EXT1, in the mice embryo using Cre-loxP system when axons of retinal ganglion cells started to extend toward the optic disk. Mice carrying transgenic gene for tamoxifen-induced Cre enzyme were bred to mice with loxP-modified EXT1 allele. To disrupt EXT1 gene during optic nerve head formation, tamoxifen was injected to the embryos carrying EXT1-floxed allele and Cre gene.

Results:: Compared with wild-type embryos, HS-deficient embryos displayed remarkable misguidance of ganglion cell axons in the retina. The disturbed morphogenesis of the optic nerve head was closely similar to the phenotype in mice lacking netrin-1, which is a HS-binding guidance molecule.

Conclusions:: HS deficiency leads to abnormal optic nerve head morphogenesis. The finding suggests that HS may regulate the function of guidance molecules in the optic disk.

Keywords: proteoglycans/glycosaminoglycans • development • optic nerve 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×