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Y. E. Osorio, K. R. Mott, E. Maguen, A. B. Nesburn, H. Ghiasi; Role of Anti-Glycoproteins D (Anti-gD) and K (Anti-gK) IgGs in Pathology of Herpes Stromal Keratitis in Human. Invest. Ophthalmol. Vis. Sci. 2007;48(13):728.
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To assess: (A) the relative impact of antibodies to HSV-1 glycoproteins on eye disease due to HSV-1 infection; (B) the composition of antibodies to 10 of the viral glycoproteins; and (C) the role of anti-gD and -gK antibodies to antibody-dependent enhancement (ADE).
In a prospective case-control study, sera from individuals with history of HSK were compared with sera from non-ocular HSV-1-seropositive and HSV-seronegative control subjects. HSV-1 neutralizing antibody titer, and type-specific IgG and IgM were measured. In addition, the presence of anti-HSV-1 gD and gK antibodies in the sera of all individuals also was determined by ELISA using gD and gK antigens. Finally, the role of anti-gD and gK-specific antibodies to ADE was investigated.
The average neutralizing antibody titers and levels of HSV-1 IgG were similar between HSK and non-HSK seropositive individuals. However, the contribution of gD to the neutralizing antibody titer in HSK sera was significantly lower than that of non-HSK seropositive individuals, despite having higher anti-gD ELISA titers. Overall, sera from HSK individuals had higher anti-gK antibody titers and induced ADE in vitro compared with non-HSK or seronegative sera. The ADE response in HSK sera was due to anti-gK antibody.
Our results suggest that: (1) sera from HSK individuals had higher anti-gD and -gK antibody titers than sera from seropositive individuals with no history of HSK despite having similar levels of neutralizing antibody titers and HSV-1 IgG; (2) HSK sera induced ADE, while sera from non-HSK individual did not induce ADE; and (3) anti-gD antibody in sera of HSK individuals contributed less to the HSV-1 neutralization antibody titer than sera from non-HSK individuals.
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