May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Firm Attachment to and Penetration of Silicone Hydrogel Contact Lenses by Representatives of Fusarium Oxysporum - F. Solani Complexes
Author Affiliations & Notes
  • S. Zhang
    Biology Dept, Georgia State University, Atlanta, Georgia
  • R. B. Simmons
    Biology Dept, Georgia State University, Atlanta, Georgia
  • D. G. Ahearn
    Biology Dept, Georgia State University, Atlanta, Georgia
  • R. D. Stulting
    Emory University, Atlanta, Georgia
  • B. L. Schwam
    Private practice, Jacksonville, Florida
  • G. E. Pierce
    Biology Dept, Georgia State University, Atlanta, Georgia
  • S. A. Crow, Jr.
    Biology Dept, Georgia State University, Atlanta, Georgia
  • Footnotes
    Commercial Relationships S. Zhang, None; R.B. Simmons, None; D.G. Ahearn, None; R.D. Stulting, None; B.L. Schwam, None; G.E. Pierce, None; S.A. Crow, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 739. doi:
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      S. Zhang, R. B. Simmons, D. G. Ahearn, R. D. Stulting, B. L. Schwam, G. E. Pierce, S. A. Crow, Jr.; Firm Attachment to and Penetration of Silicone Hydrogel Contact Lenses by Representatives of Fusarium Oxysporum - F. Solani Complexes. Invest. Ophthalmol. Vis. Sci. 2007;48(13):739.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To investigate the relative capacities of representatives of the Fusarium oxysporum - F. solani complexes to attach to and to penetrate silicone hydrogel soft contact lenses.

Methods:: Silicone hydrogel (SH) soft contact lenses were exposed to conidial suspensions of isolates of the F. oxysporum - F. solani complexes in vitro (104 conidia/ml in phosphate-buffered saline). The lenses were incubated with shaking at ambient temperatures and examined after rinsing in a multipurpose contact lens solution with light microscopy. Selected lenses were examined with scanning electron microscopy.

Results:: Isolates of both species within the complex firmly attached to and penetrated SH lenses. The degree of attachment and time required for penetration into the lenses varied by strain and substratum. The fungal morphologies (e.g. penetration pegs) of cultures on the lenses in PBS were similar to those observed on lenses from patients with Fusarium keratitis associated with ReNu® with MoistureLoc®. Hyphal elements were observed on lenses after rinsing with MPS, and a few elements were occasionally present following a manual cleaning regimen.

Conclusions:: Representatives of F. oxysporum - F. solani complexesattach firmly to SH lenses, and may, in rare instances, penetrate the lens matrix. This may, in part, explain the recent outbreak of Fusarium keratitis associated with contact lens wear. Use of a manual cleaning-disinfection procedure is recommended to reduce the risk of potential transmission of fungal elements from a lens to the eye.

Keywords: contact lens • fungal disease 
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