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J. L. Alio, C. Ferrer, J. L. Rodriguez-Prats; Evaluation of Adherence of S. epidermidis on Different IOLs. Invest. Ophthalmol. Vis. Sci. 2007;48(13):740.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the quantity of Staphylococcus epidermidis cells adhered to different intraocular lens materials.
S. epidermidis strain N890074 was incubated for 1 hour or 24 hours on different IOLs: Kelman Duet (silicone), Alcon MZ60BD (PMMA), Alcon SA60AT (acrylate) or Alcon MA60BM (acrylate-metacrilate). Three groups of study: in group I, IOLs were incubated with S. epidermis only; in group II IOLs were immersed in ophthalmic viscosurgical solution (Hydroxypropyl Methylcellulose, Celoftal®) and washed in PBS before incubation with S. epidermidis; in group III, IOLs were incubated in Celoftal® and S. epidermidis (1:1). IOLs were examined by scanning electron microscopy (SEM), to evaluate bacterial biofilm formation in each group.
Among the four IOLs tested, the lower amount of attached cells was observed on the silicone IOLs. Biofilm production was significantly different between silicone and the rest of lenses at 1 hour and 24 hours (p<0.05). The bacterial populations were no significantly different between IOLs in the three groups at 1 hour of incubation but were significantly different between the group I and group II or III at 24 hours. Between groups II and III were no significant differences were observed at any time point.
S. epidermidis level of adherence was in the following order: metacrilate (Alcon MA60BM) > acrilate (Alcon SA60AT ) > PMMA (Alcon MZ60) > silicone (Kelman Duet). On the other hand, ophthalmic viscosurgical solution (Hydroxypropyl Methylcellulose, Celoftal®) seems to avoid adherence of cells to the surface lenses.
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