May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Antibiotic Susceptibility of Conjunctival Bacterial Isolates From Refractive Surgery Patients
Author Affiliations & Notes
  • J. Chung
    Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Republic of Korea
  • K. Seo
    Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Republic of Korea
  • D. Yong
    Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea
  • F. S. Mah
    Charles T. Campbell Ophthalmic Microbiology Laboratory, University of Pittsburgh, Pittsburgh, Pennsylvania
  • T.-i. Kim
    Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Republic of Korea
  • H. Lee
    Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Republic of Korea
  • E. Kim
    Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Republic of Korea
  • C. Kim
    Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea
  • J. Kim
    Balgensesang Ophthalmology Clinic, Seoul, Republic of Korea
  • S. Kim
    Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships J. Chung, None; K. Seo, None; D. Yong, None; F.S. Mah, None; T. Kim, None; H. Lee, None; E. Kim, None; C. Kim, None; J. Kim, None; S. Kim, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 741. doi:
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      J. Chung, K. Seo, D. Yong, F. S. Mah, T.-i. Kim, H. Lee, E. Kim, C. Kim, J. Kim, S. Kim; Antibiotic Susceptibility of Conjunctival Bacterial Isolates From Refractive Surgery Patients. Invest. Ophthalmol. Vis. Sci. 2007;48(13):741.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To determine the in vitro antibiotic susceptibility patterns of conjunctival bacterial flora isolated preoperatively from patients undergoing refractive surgery.

Methods:: One hundred five eyes from 105 patients scheduled for refractive surgery between September 2005 and January 2006 were studied. Among 105 patients, 71 (67.6%) LASIK using femtosecond laser, 24 (22.9%) conventional LASIK, 8 (7.6%) LASEK, and 2 (1.9%) phakic intraocular lens implantations were performed. Preoperative conjunctival swab samples were directly inoculated to culture media at bedside before topical anesthetic or antibiotic application. Blood agar, chocolate agar, thioglycolate broth, sabouraud dextrose agar, and Ogawa media were used for bacterial, fungal and mycobacterial culture. Minimum inhibitory concentrations (MICs) of ofloxacin (OFX), levofloxacin (LEV), gatifloxacin (GAT), moxifloxacin (MOX), gemifloxacin (GEM) and other commonly used antibiotics were determined using E-test.

Results:: From 105 patients, 74 (85%) coagulase-negative staphylococci (CNS), 2 S. aureus, 1 S. pneumoniae and 5 gram-negative bacilli were isolated. No fungus and mycobacterium were isolated. MIC90s (mg/mL) of OFX, LEV, GAT, MOX, and GEM for methicillin-susceptible CNS (n=46) were 0.5, 0.19, 0.094, 0.047, and 0.023. And those for methicillin-resistant CNS (n=27) were 32, 4, 1, 0.5, and 0.25, respectively.

Conclusions:: GEM, MOX and GAT were the most active against conjunctival bacteria from refractive surgery patients. However resistance against lower generation fluoroquinolone (OFX and LEV) is increasing among methicillin-resistant CNS.

Keywords: antibiotics/antifungals/antiparasitics • refractive surgery • Staphylococcus 
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