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J. M. Merayo-Lloves, T. Blanco-Mezquita, L. Ibares-Frias, R. Cantalapiedra, A. Aalvarez-Barcia; Evaluation Of Topical Administration T1225 (azithromycin Oil Based Eye Drops) In Corneal Wound Healing After Refractive Surgery In An Experimental Model. Invest. Ophthalmol. Vis. Sci. 2007;48(13):783.
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The expected benefit of topical application of T1225 eye drops (Laboratories Thee, France) in corneal refractive surgery is antimicrobial prophylaxis of the ocular surface. Therefore it makes sense to evaluate the tolerance of this topical drug in various models of surgically-induce corneal injuries like surface ablation model (PRK) and LASIK .As these eye drops are oil-based we wonder whether this slippery characteristic might negatively interfere with the good achievement of the cornea stromal and epithelial flap and corneal wound healing after injury.The purpose of this project is to evaluate the effect of the antibiotic eye drops T1225 in corneal wound healing after refractive surgery in hens
60 adult Lothmann Braun hens were divided in 6 groups of 10 animals. Group 1 (unmanipulated), group 2 (PRK) and group 3 (Lasik) were given 20 Microliters of saline eye drops 3 days prior to surgery and 3 days after surgery bid. Groups 4, 5, and 6 underwent the same surgery as 1, 2, and 3 and were treated with T1225 (1.5% azithromycin) in the same fashion.Outcome was measured by clinical parameters on days 1 to 6 (corneal epithelial closure, clinical grade of haze and corneal thickness). Cell biology parameters were evaluated on day 6 post opterative: light histology, apoptosis (Tunnel) cell proliferation (Brdu Staining), migration and differentiation (myofibroblast formation).Animal facilities
T 1225 was well tolerated with no clinical differences (time of closure, haze and pachymetry) for PRK and Laic groups compared with the saline treated animals. Animals that underwent corneal surgery developed apoptosis, proliferation and myofibroblast formation (more in the PRK groups with statistical significant differences) but there were no differences among the groups treated with saline or T1225 for the same type of corneal surgery
Topical administration of T1225 oil based azithromycin eye drops is well tolerated in the non manipulated animals and hens treated with corneal refractive surgery (PRK and Lasik). There were no differences in wound healing and flap adherence.
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