May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Matrix Metalloproteinases in Human Choroidal Neovascular Membranes Excised Following Verteporfin Photodynamic Therapy
Author Affiliations & Notes
  • O. Tatar
    Ophthalmology, University Eye Hospital, Tubingen, Germany
  • K. Shinoda
    Ophthalmology, Laboratory of Visual Physiology, National Institute of Sensory Organs, Tokyo, Japan
  • A. Adam
    Department of Pathology, Tubingen, Germany
  • V. Boeyden
    Ophthalmology, AZ Saint-Augustinus Hospital, Wilrijk, Belgium
  • G. Pertile
    Ophthalmology, Eye Hospital, Negrar, Italy
  • S. Bopp
    Ophthalmology, Eye Clinic Universitätsallee, Bremen, Germany
  • E. Yoeruek
    Ophthalmology, University Eye Hospital, Tubingen, Germany
  • C. Eckardt
    Ophthalmology, Augenklinik der Staedtischen Kliniken, Frankfurt am Main, Germany
  • K. U. Bartz-Schmidt
    Ophthalmology, University Eye Hospital, Tubingen, Germany
  • S. Grisanti
    Ophthalmology, University Eye Hospital, Tubingen, Germany
  • Footnotes
    Commercial Relationships O. Tatar, None; K. Shinoda, None; A. Adam, None; V. Boeyden, None; G. Pertile, None; S. Bopp, None; E. Yoeruek, None; C. Eckardt, None; K.U. Bartz-Schmidt, None; S. Grisanti, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1465. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      O. Tatar, K. Shinoda, A. Adam, V. Boeyden, G. Pertile, S. Bopp, E. Yoeruek, C. Eckardt, K. U. Bartz-Schmidt, S. Grisanti; Matrix Metalloproteinases in Human Choroidal Neovascular Membranes Excised Following Verteporfin Photodynamic Therapy. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1465.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose:: To evaluate the expression of the proangiogenic matrix metalloproteinases (MMP) 2 and 9 at distinct intervals after verteporfin photodynamic therapy (PDT) in human choroidal neovascular membranes (CNV) secondary to age-related macular degeneration (AMD).

Methods:: Retrospective review of an interventional case series of forty-nine patients who underwent removal of CNV. Twenty-six patients were treated with PDT 3 to 383 days prior to surgery. Twenty-three CNV without previous treatment were used as controls. CNV were stained for CD34, cytokeratin18, endostatin, MMP-2 and MMP-9 by immunohistochemistry.

Results:: CNV without previous therapy disclosed MMP-2, MMP-9 in RPE-Bruch’s membrane, vessels and stroma in different intensities. Three days after PDT, MMP-9 expression was significantly weaker in stroma (p=0.0019). A significantly reduced expression was also found for endostatin in vessels (p=0.0005). At longer post-PDT intervals, a significant increase of MMP-9 expression in stroma (p=0.0373) and of endostatin expression in RPE-Bruch’s membrane (p=0.02), vessels (p=0.005) and stroma (p=0.0007) were disclosed. No significant changes in MMP-2 expression were detected.

Conclusions:: PDT induced an early, temporary decrease in MMP-9 and endostatin expression. At longer intervals, MMP-9 increase is possibly associated with the angiogenic process responsible for recurrence after PDT. MMP-9, however, acts as a double-edged sword by concomitant induction of endostatin, an endogenous inhibitor of angiogenesis.

Keywords: age-related macular degeneration • photodynamic therapy • choroid: neovascularization 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.