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L. Federici, F. Chavane, G. Masson, B. Ridings, J. Conrath; Caracterization of a New Model of Experimental Choroidal Neovascularization in the Non Human Primate. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1472.
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The induction of and the angiographic, histologic and OCT study of experimental neovascularization in the non human primate can allow a better comprehension and thus better treatment of exsudative neovascular age-related macular degeneration (AMD).
6 female baboons from 10 to 15 years of age were studied. A diode laser (wavelength 810 Nm) was used to induce rupture of Bruch’s membrane. The laser beam is transmitted using and indirect head-worn ophtalmoscop and focused on the retina using a 28 dioptre lens allowing simultaneous fundus visualization.Follow-up fundus and fluorescein angiography examinations were conducted each week postlaser. 6 weeks after laser impacts, the primates were enucleated and eyes underwent OCT and histologic examinations.
The optimal parameters were established with impacts ranging from 900 to 970 mW, causing vaporization bubbles by Bruch’s membrane rupture. Impact duration was 150 milli seconds and spot size is estimated at approximately 500 micrometers. The new vessels appeared in our study between the second and the third week post laser. On late shots of the angiography sequence we see a hyper-fluorescent lesion, with leakage and greater size at early shots. The histologic examination confirms the already known fact that the response to laser aggression described as new vessels is in fact a fibrous tissue containing vessels from the simple capillary to a well-developed vascular network. Fibro-vascular proliferation is represented on OCT by a thickening of the RPE/Bruch’s membrane, a small detachment of the pigment epithelium associated with its effects shown by serous retinal detachment or cystoid macular oedema.
Point of considering angiographic, OCT and histologic examinations, the neovascular response to laser aggression described herein in our model is similar to that which is seen in many human diseases and especially exsudative neovascular age-related macular degeneration.For this reason, its interest is major since it will enable us to test the effectiveness of anti angiogenic molecules yet unused in man or used without proof of their inocuity, or of their effectiveness.
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