May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Dark-Adaptation ERG Recovery in Different Mice Strains
Author Affiliations & Notes
  • R. T. Tzekov
    Drug Discovery Division, Acucela Inc, Bothell, Washington
  • N. Matson
    Drug Discovery Division, Acucela Inc, Bothell, Washington
  • D. McGee
    Drug Discovery Division, Acucela Inc, Bothell, Washington
  • R. Kubota
    Drug Discovery Division, Acucela Inc, Bothell, Washington
  • Footnotes
    Commercial Relationships R.T. Tzekov, None; N. Matson, None; D. McGee, None; R. Kubota, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1520. doi:
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      R. T. Tzekov, N. Matson, D. McGee, R. Kubota; Dark-Adaptation ERG Recovery in Different Mice Strains. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1520.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: The purpose of this work was to compare the parameters of the electroretinogram (ERG) recovery during the course of a dark-adaptation after moderate intensity photobleach in three strains of mice.

Methods:: Full-field ERGs were recorded from male CD-1, BALB/c and C57BL/6 mice at 8 to 12 weeks of age (weight 20 - 45 g). Two stimulation intensities were used throughout the recording: 0.01 cd.s/m2 and 1 cd.s/m2. After recording baseline dark-adapted ERG responses, ~400 lux white light was presented for 45 sec to provide ~45% photobleaching of rhodopsin. ERG recordings from both intensities were recorded every 5 min up to 45 min after bleach. A-wave was measured at a fixed time point after flash, whereas b-wave amplitudes were measured from the trough of the a-wave to the peak of the b-wave after elimination of the superimposed oscillatory potentials (OP) with digital filtering.

Results:: The amplitude of the a-wave was measurable only at 1 cd.s/m2 intensity and also exhibited full recovery (118 to 143%) without any strain difference. The amplitude of the b-wave fully recovered in all three strains by the end of the 45 minute follow-up period (101-113% for 0.01 cd.s/m2 and 111-126% for 1 cd.s/m2) and there were no significant differences between the strains. The coefficient of variation (CV) for a- and b-wave amplitude recovery was considerably lower in C57BL/6 mice (5-21%) compared to either CD-1 (35-38%) or BALB/c mice (43-48%). Dark-adapted a- and b-wave amplitudes did not differ significantly between the strains.

Conclusions:: Despite the disparity in ocular pigmentation (lack of retinal/choroidal melanin in CD-1 and BALB/c vs. presence of melanin in C57BL/6), the amplitude of the ERG a- and b-waves fully recovered in all three strains at 45 min after moderate bleach. However, the variability of the response was less in. pigmented strains than in albinos.

Keywords: electrophysiology: non-clinical • electroretinography: non-clinical • photoreceptors: visual performance 

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