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D. C. Chung, T. Rex, V. Lee, W. Tang, Z. Wei, E. Oh, A. Swaroop, J. Bennett; Somatic in utero Gene Delivery to E14 Nrl-/- Photoreceptors Induces Rod Specific Protein Expression. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1691.
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Transgenic Nrl-/- mice underwent timed pregnancies. At embryonic day 14 (E14), the left subretinal space was injected with an adeno-associated virus encoding the wildtype nrl gene (AAV. CMV2/5.nrl). Non-injected contralateral eyes served as controls. At postnatal day 45 (P45) electroretinograms (ERG) and immunohistochemical analysis for rod-photoreceptor specific proteins were performed.
Experimental and control eyes exhibited no significant ERG differences. However, histological sections exhibited rhodopsin and transducin positive photoreceptors cells.
In utero gene delivery can alter isolated photoreceptor cell differentiation from a cone pathway to a rod pathway evidenced by the expression of rod-specific proteins. Earlier delivery may increase the number of cells with a rod photoreceptor fate. Future visual behavioral analysis will also evaluate rod cell function.<br /
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