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Y.-J. Jo, Y. Oshima, K.-H. Sonoda, T. Yoshimura, K. Hijioka, T. Fujimoto, T. Ishibashi; Continuous Expression of VEGF in Photoreceptors Causes Severe Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1731.
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Increased expression of vascular endothelial growth factor (VEGF) in the photoreceptors is sufficient to stimulate sprouting of neovascularization from the deep capillary bed of the retina, but not from the superficial retinal capillaries or the choriocapillaris. In this study, we investigated the effect of the increased VEGF expression in the deep retina on laser-induced choroidal neovascuralization (CNV) using transgenic mice with increased expression of human VEGF in photoreceptors (rho/VEGF transgenic mice).
Adult rho/VEGF transgenic mice were used to investigate the effect of increased expression of VEGF in photoreceptors on the laser-induced CNV model. The CNV was assessed on days 14 and 28 in rho/VEGF mice and C57BL/6 control mice by histological examination and image analysis of flat mounts from mice perfused with fluorescein-labeled dextran. RT-PCR and microbead-based multiplex ELISA from the eyes of both groups of mice were performed twenty-four hours after laser treatment.
Rho/VEGF transgenic mice had significantly larger CNV than C57BL/6 mice both on days 14 and 28. In C57BL/6 mice, the CNV on day 28 significantly regressed compared to day 14, however in rho/VEGF transgenic mice, the CNV increased significantly.
These data suggested that continuous increased expression of VEGF enhances the formation of CNV and prevents the normally observed regression.
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