May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Role of EMMPRIN (cd147), An Inducer of Matrix Metalloproteinases Synthesis During Corneal Remodelling
Author Affiliations & Notes
  • E. E. Gabison
    Cornea, Fondation Ophtal A de Rothschild, Hopital bichat AP-HP, Paris, France
  • E. Huet
    Cornea, Fondation Ophtal A de Rothschild, CNRS UMR 7149, Paris, France
  • B. Valée
    Cornea, Fondation Ophtal A de Rothschild, CNRS UMR 7149, Paris, France
  • D. Szul
    Cornea, Fondation Ophtal A de Rothschild, CNRS UMR 7149, Paris, France
  • F. Verrecchia
    Hôpital St Louis, INSERM U697, Paris, France
  • T. Hoang-Xuan
    Cornea, Fondation Ophtal A de Rothschild, Hopital bichat AP-HP, Paris, France
  • S. Menashi
    Cornea, CNRS UMR 7149, Paris, France
  • Footnotes
    Commercial Relationships E.E. Gabison, None; E. Huet, None; B. Valée, None; D. Szul, None; F. Verrecchia, None; T. Hoang-Xuan, None; S. Menashi, None.
  • Footnotes
    Support Fondation de l'Avenir
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1960. doi:
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      E. E. Gabison, E. Huet, B. Valée, D. Szul, F. Verrecchia, T. Hoang-Xuan, S. Menashi; Role of EMMPRIN (cd147), An Inducer of Matrix Metalloproteinases Synthesis During Corneal Remodelling. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1960.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: We previously described EMMPRIN, the metalloproteinase inducer, in the cornea and demonstrated its up-regulation during corneal healing process (E. E. Gabison et al. Am J Pathol 2005). In this study we investigated its role during corneal remodelling.

Methods:: EMMPRIN expression was analyzed in normal and ulcerated human corneas, as well as in stromal cells in culture using confoncal microscopy, immuno-blots and real-time PCR.

Results:: In normal cornea EMMPRIN was predominantly expressed in the epithelium but was markedly induced in the anterior stroma of ulcerated corneas. This coincided with MMP-2 induction which colocalized with EMMPRIN at the epithlio-stromal boundary. In an in-vitro coculture system we demonstrated the induction and colocalization of EMMPRIN and MMP-2 in the fibroblasts at the interface with epithelial cells. Direct contact of fibroblasts with EMMPRIN-containing purified epithelial cell membranes also induced MMP-1, MMP-2 and EMMPRIN and this was inhibited by a blocking anti-EMMPRIN antibody. Additionnaly, EMMPRIN and MMP-2 were also induced by TGF-b in corneal fibroblasts in culture

Conclusions:: EMMPRIN up-regulation in the stroma during corneal wound healing and its modulation by TGFb and cell-cell interactions suggest an important role in corneal remodelling

Keywords: wound healing • cornea: stroma and keratocytes • proteolysis 
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