May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Functional Role of Smad4 in Lens Formation
Author Affiliations & Notes
  • K. Kawai
    Ophthalamology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • F. F. Zhuang
    Ophthalamology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • Y.-H. Liu
    Ophthalamology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships K. Kawai, None; F.F. Zhuang, None; Y. Liu, None.
  • Footnotes
    Support NIH Grant EY015417
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1999. doi:
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      K. Kawai, F. F. Zhuang, Y.-H. Liu; Functional Role of Smad4 in Lens Formation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1999.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To clarify functional roles of downstream mediators of BMP (Bone morphogenic proteins) signaling during the early stages of lens morphogenesis, we selectively inactivated the Smad4 gene in the developing lens.

Methods:: Floxed Smad4 animals were crossed to Le-Cre transgenic animals. Embryos at E9.5, E10.5, E12.5 and eyes from adult stages were used forhistological analysis.

Results:: We observed anomalies not only in lens and corneal formation but also in the vitreous and posterior capsule:•The size of lens was reduced by apoptosis.•The corneal epithelium was loosely packed.•The number of mesenchymal cells in vitreous was increased.•A mass of cells attached to the posterior capsule of lens was observed.

Conclusions:: Smad4 is required for proper lens formation.

Keywords: apoptosis/cell death • cornea: epithelium • retinal development 
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