May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Lens Pathology in Rescued Perlecan-Knockout Mice
Author Affiliations & Notes
  • I. Masuda
    Dept of Ophthalmology, Okayama Univ Med School, Okayama City, Japan
    Section of Retinal Diseases & Therapeutics,
    National Eye Institute, Bethesda, Maryland
  • Y. Takada
    Institue on Deafness and Other Communication Disorders, Bethesda, Maryland
  • T. Igarashi
    Section of Retinal Diseases & Therapeutics,
    National Eye Institute, Bethesda, Maryland
  • S. Zigler
    Lens and Cataract Biology,
    National Eye Institute, Bethesda, Maryland
  • Y. Yamada
    National Institute of Dental and Craniofacial Research, Bethesda, Maryland
  • K. Csaky
    Section of Retinal Diseases & Therapeutics,
    National Eye Institute, Bethesda, Maryland
  • Footnotes
    Commercial Relationships I. Masuda, None; Y. Takada, None; T. Igarashi, None; S. Zigler, None; Y. Yamada, None; K. Csaky, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2001. doi:
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      I. Masuda, Y. Takada, T. Igarashi, S. Zigler, Y. Yamada, K. Csaky; Lens Pathology in Rescued Perlecan-Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2001.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: Perlecan is a major heparan sulfatrte proteoglycan (HSPG) found in basement membranes (BMs) and connective tissue. Heparan sulfate(HS) side cahins in the lens capsule has been reported to have a structural function and be essential in the insulation of the lens. The aim of this study is to examine the role for the central core protein of perlecan in lens development.

Methods:: Perlecan-knock-out mice(prl-/prl-) were rescued by coexpression of perlecan under the control of the mouse type II collagen gene regulatory regions. Paraffin section of eyes from Rescued Perlecan-Knockout mice were stained with hematoxylin-eosin,PAS and In-Situ TUNEL assays. Frozen sentions of eyes were stained with perlecan antibody and expression of adhesion molecules ( cadherin , integrin). ). Lenses from wild-type mice and perlecan-knockout mice were kept in organ culture with either contrtol(DMEM) or perlecan(DMEM+perlecan) for 1,3,7 days. The cataract formation was examined using a dissecting microscope.

Results:: Immunostaining for perlecan confirmed the presence of perlecan in lens in prl+/prl+ and prl+/prl- mice but not rescued prl-/prl- mice. Histology of the lens showed evidence of lens degeneration with detachment of the lens epithelium.

Conclusions:: The core protein of perlecan play a critical role in lens development, absence of which results in lens epithelial and capsular changes.

Keywords: extracellular matrix • cataract • immunohistochemistry 

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