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M. Caro Magdaleno, G. A. Peyman; Lissamine Green B Staining of Lens Capsule and Internal Limiting Membrane (ILM). Invest. Ophthalmol. Vis. Sci. 2007;48(13):2024.
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To study the use of Lissamine Green B (LGB) in vitro for staining of the lens capsule and for staining of the ILM in dye-assisted capsulorrexis and its potential application in macular hole surgery.Vital dyes such as Trypan blue and indocyanine green (ICG) have been used to assist in the anterior and posterior segment surgery. Lissamine Green B (LGB), a vital stain, is in clinical use for its ability to stain the damaged corneal and conjunctival cells in dry eye.
18 freshly enucleated pig eyes were obtained from the slaughter house. The eyes were divided into two groups. The first group was used to stain the lens capsule using various concentrations of LGB: (0.05%, 0.1%, 0.15 % and 0.25 %) prior to capsulorrexis. 0.05 ml of each solution was injected in the anterior chamber. Then a routine capsulorrexis was done and photographed. In the second group we removed the cornea, the lens, and the vitreous of these eyes. We then applied a few micro-liters of 0.05 % and 0.1 % LGB solution on the ILM under air. After a few seconds the stain was washed away with saline solution and the ILM wad photographed.
Although the lens capsule was stained well with 0.05%-0.25% concentrations used, the 0.1% concentration appeared very adequate. Similar results were achieved for staining of the ILM.
LGB solution stained the lens capsule and the ILM successfully.
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