May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
The Use of Fugo Blade for Chorioretinal Biopsy
Author Affiliations & Notes
  • E. Letko
    Ophthalmology, Casey Eye Institute, Portland, Oregon
  • D. J. Wilson
    Ophthalmology, Casey Eye Institute, Portland, Oregon
  • Footnotes
    Commercial Relationships E. Letko, None; D.J. Wilson, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2252. doi:
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      E. Letko, D. J. Wilson; The Use of Fugo Blade for Chorioretinal Biopsy. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2252.

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Abstract

Purpose:: To evaluate wound repair after chorioretinal biopsy using the FUGO blade, or conventional diathermy.

Methods:: Transvitreal chorioretinal biopsy was performed on a rabbit model. The FUGO blade was used to excise a chorioretinal specimen in two rabbits (four eyes) and endodiathermy was used as control in one rabbit (two eyes). After chorioretinal biopsy in one eye each rabbit was followed for five weeks and at that point a non-surviving chorioretinal biopsy was performed in a fellow eye. The eyes were enucleated and fixed in 10 % formalin. Four micron sections were cut and stained with hematoxylin and eosin and examined with light microscopy.

Results:: There was some subjective greater ease of doing the biopsy with the FUGO blade. Histopathologic stains showed that the architecture of the retina and choroid at the margin of the biopsy site was well preserved, with either modality. Partial thickness scleral injury was noted in the two eyes that underwent non-surviving chorioretinal biopsy, with the FUGO blade.

Conclusions:: These results suggest that the damage to the chorioretinal tissue at the margin of the biopsy site was similar when either FUGO blade or endodiathermy was used. The FUGO blade induced more thermal damage to the underlying sclera when compared to endodiathermy, but refinement of instrument design could alleviate this limitation.

Keywords: choroid • retina • chorioretinitis 
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