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A. Iga Rashi, N. Okada, Y. Satake, M. Dogru, K. Fukagawa, J. Shimazaki, K. Tsubota, H. Fujishima; The Change of Prostaglandins,and Cysteinyl-leukotriene Concentrations in the Tears of Patients With Severe Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2310.
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Prostaglandins(PGs)and cysteinyl-leukotrienes(Cys-LTs) have been reported to mediate various inflammatory responses. However, studies detailing the levels of PGs and Cys-LTs in tears of patients with atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) have not yet been extensively reported. In this study, we examined the concentration of PGD2 , PGJ2, PGE2,and Cysteinyl-Leukotriene in tears from patients with severe allergic conjunctivitis.
Tear samples were collected from normal control subjects and from patients with VKC and AKC. 20 µL of tears were collected by capillary tubes; samples were centrifuged 10000rpm for 5 minutes. The supernatants were then stored at -20°C until concentration measurements by ELISA and EIA.The concentrations of PGD2(12eyes),PGE2(16eyes),PGJ2(10eyes),Cysteinyl-Loukotrienes(16eyes) were measured using ELISA and EIA.The concentrations were then compared between patients with AKC, VKC and normal control subjects using the t-test for statistical comparisons.Informed consents were obtained from all patients.This study followed the Tenets of the Declaration of Helsinki.
The concentrations of PGD2, PGE2, PGJ2, and cysteinyl-Leukotriene were found to be significantly higher (P<0.05) in patients with allergic conjunctivitis than in normal control subjects. However there were no statistically significant differences between patients with AKC and VKC.
Both PGs and LTs appear to significantly contribute to the inflammatory response and development of severe allergic conjunctivitis.
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