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V. C. Mody, Jr., M. Kakar, M. Ayala, L. Meyer, X. Dong, J. Wang, Y. Zhang, C. Bucht, S. Löfgren, P. G. Söderberg; Ultraviolet Radiation-B-(UVR-B)-Induced Cataract in the Pigmented Guinea Pig, a Very UVR-B-Resistant Animal Species. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2421.
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To investigate resistance to UVR-B-induced cataract development based on short-term cataract development and measurement of a threshold safety limit in the pigmented guinea pig.
Evolution: Twenty-four pigmented guinea pigs were exposed to 80 kJ/m2 UVR-B for sixty minutes under anaesthesia in vivo and thereafter sacrificed at 1, 2, 4, and 8 days after exposure. Degree of cataract was determined by quantitative measurement of forward light scattering in the isolated lenses. Safety limit: Thirty pigmented guinea pigs were exposed to 0-84.9 kJ/m2 UVR-B and thereafter sacrificed at one day after exposure. The data were fit to a second order polynomial using regression. MTD, maximum tolerable dose for avoidance of UVR-B-induced cataract, was determined for the pigmented guinea pig.
All non-exposed lenses from both studies were devoid of cataract. Anterior subcapsular cataract developed in all UVR-B-exposed lenses. A maximum degree of in vivo forward light scattering developed at one day after exposure and remained constant up to eight days after exposure. MTD for the pigmented guinea pig was 69.0 kJ/m2 UVR-B.
One day is an appropriate time interval for study of the safety limit for UVR-B-induced cataract in the pigmented guinea pig. The MTD for avoidance of UVR-B-induced cataract in the pigmented guinea pig is more than ten times the threshold obtained by Pitts et al. in the pigmented rabbit. MTD is an appropriate method for estimation of toxicity for UVR-B-induced cataract in the guinea pig. The pigmented guinea pig is much less sensitive to UVR-B exposure than the pigmented rabbit, pigmented rat, and pigmented mouse.
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