May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Dose-Response Relationship for A-Tocopherol Prevention of Ultraviolet Radiation Induced Cataract in Rats
Author Affiliations & Notes
  • J. Wang
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
    Ophthalmology, Shandong University, Jinan, China
  • X. Dong
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • M. Ayala
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • S. Löfgren
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • V. C. Mody, Jr.
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • L. Meyer
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • Y. Zhang
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • C. Bucht
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • P. G. Söderberg
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • Footnotes
    Commercial Relationships J. Wang, None; X. Dong, None; M. Ayala, None; S. Löfgren, None; V.C. Mody, None; L. Meyer, None; Y. Zhang, None; C. Bucht, None; P.G. Söderberg, None.
  • Footnotes
    Support Swedish Research Council, project K2006-74X-15035-03-2, Karolinska Institutet Research Foundation, Gamla Tjänarinnor Foundation, Swedish Radiation Protection Institute
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2422. doi:
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      J. Wang, X. Dong, M. Ayala, S. Löfgren, V. C. Mody, Jr., L. Meyer, Y. Zhang, C. Bucht, P. G. Söderberg; Dose-Response Relationship for A-Tocopherol Prevention of Ultraviolet Radiation Induced Cataract in Rats. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2422.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To investigate if the preventive effect of α-tocopherol on ultraviolet radiation (UVR) induced cataract in an in vivo animal model is dose-dependent.

Methods:: Five groups of twenty, six-week-old female albino Sprague Dawley rats were used in present study. One group without any α- tocopherol feeding was the control group, while the other four groups received 5, 25, 50, and 100 IU/day of α- tocopherol by oral administration respectively. After 4 weeks of feeding, each rat was unilaterally exposed to 8 kJ/m2 UVR-300 nm for 15 min. At 1 week after exposure to UVR, the rats were sacrificed, the lenses were extracted and photographed, and forward light scattering was measured.

Results:: The UVR-exposed lenses in the α-tocopherol fed groups developed superficial and slight equatorial cataract, whereas lenses in the control group developed cortical and dense equatorial opacities. Forward light scattering in lenses from the α-tocopherol-supplemented rats was lower than in lenses from the control group. Lenses in rats that had been fed 25 IU/day of α-tocopherol expressed the lowest intensity of forward light scattering. Lenses in rats that had been fed 50 or 100 IU/day expressed no significant difference in forward light scattering than lenses from rats that had been fed 25 IU/day. There was no difference in lens opacity or forward light scattering among non-exposed lenses.

Conclusions:: Oral administration of α-tocopherol to rat protects the lens against UVR-induced cataract. The protection is dose dependent and saturable.

Keywords: cataract • antioxidants • radiation damage: light/UV 
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