May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Alpha-Crystallins Modulate P53 Phosphorylation Status to Attenuate Uva-Induced Apoptosis
Author Affiliations & Notes
  • J.-P. Liu
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • J. Qin
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Q. Yan
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • H.-G. Chen
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • D. W. Li
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships J. Liu, None; J. Qin, None; Q. Yan, None; H. Chen, None; D.W. Li, None.
  • Footnotes
    Support Supported by 1R01 EY15765, University of Nebraska Medical Center, and the Lotus Scholar Professorship funds from Hunan Province Government and Hunan Normal University.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2425. doi:
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      J.-P. Liu, J. Qin, Q. Yan, H.-G. Chen, D. W. Li; Alpha-Crystallins Modulate P53 Phosphorylation Status to Attenuate Uva-Induced Apoptosis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2425.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: UVA-induced apoptosis is linked to cataractogenesis of the ocular lens. The ocular lens normally guards apoptosis of the lens epithelial cells through the function of the lens structure proteins, alpha-crystallins. In our previous studies, we have demonstrated that alpha-crystallins can negatively regulate UVA-induced apoptosis through suppression of the ERK-mediated pathway and activation of the AKT signaling pathway. In the present study, we present evidence to show that alpha-crystallins can modulate the phosphorylation status of the tumor suppressor, p53 to antagonize UVA-induced apoptosis.

Methods:: UVA was used to irradiate human lens epithelial cells stably expressing GFP vector, HαA-GFP, and HαB-GFP fusion proteins. Western blot analysis was used for detection of p53 phosphorylation status. Hochst staining was used for apoptosis assay.

Results:: Human lens epithelial cells expressing either alphaA- or alphaB-crystallin are substantially resistant to UVA-induced apoptosis. UVA-induces distinct phosphorylation of p53 at Ser-15 in vector-transfected cells. However, in alphaA- or alphaB-crystallin-transfected cells, phosphorylation of p53 at Ser-15 was distinctly attenuated.

Conclusions:: Alpha-crystallins prevent UVA-induced apoptosis at multiple signaling transduction pathways.

Keywords: cataract • cell survival • apoptosis/cell death 
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