May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Somatostatin Receptor (sst1- 5) Activation Leads to a Decrease in Proliferation of Human Retinal Pigment Epithelium (RPE) Cells in Culture
Author Affiliations & Notes
  • T. G. Papadaki
    University of Crete, Faculty of Medicine, Heraklion, Greece
    Ophthalmology,
    Pharmacology,
  • M. Tsilimbaris
    University of Crete, Faculty of Medicine, Heraklion, Greece
    Ophthalmology,
  • I. Pallikaris
    University of Crete, Faculty of Medicine, Heraklion, Greece
    Ophthalmology,
  • K. Thermos
    University of Crete, Faculty of Medicine, Heraklion, Greece
    Pharmacology,
  • Footnotes
    Commercial Relationships T.G. Papadaki, None; M. Tsilimbaris, None; I. Pallikaris, None; K. Thermos, None.
  • Footnotes
    Support M. Manasaki Scholarship of the University of Crete; Hellenic Ministry Of Education Grant (Pythagoras)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2518. doi:
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      T. G. Papadaki, M. Tsilimbaris, I. Pallikaris, K. Thermos; Somatostatin Receptor (sst1- 5) Activation Leads to a Decrease in Proliferation of Human Retinal Pigment Epithelium (RPE) Cells in Culture. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2518.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To investigate the differential effect of somatostatin (SRIF) and its receptor-specific analogues on the proliferation of human RPE cells in culture.

Methods:: D407 cells were employed for all studies performed. MTT assay, APOpercentageTM apoptosis assay and trypan blue exclusion method were performed to study the cytostatic, apoptotic and necrotic effects of selective ligands of all somatostatin receptor subtypes (sst 1-5).

Results:: SRIF decreased cell proliferation in a concentration-dependent manner (1x10-10-1x10-4M). Similarly, activation of the five somatostatin receptor subtypes (sst1-5) mimicked the SRIF effect and decreased cell proliferation in a concentration-dependent manner. The decrease in cell number was due to cytostatic signaling (via sst 2, 3, 4 activation), apoptotic (via sst1 and sst5 activation) and cytotoxic effects at the highest concentration of ligands used (10-4M; mainly sst1 and sst5 activation). Sodium orthovanadate, a protein tyrosine phoshatases (PTPs) inhibitor, reversed the antiproliferative effects of SRIF.

Conclusions:: This study provides novel information regarding the cytostatic, apoptotic and / or cytotoxic effects of SRIF in hRPE cells. These distinct signaling mechanisms are due to the differential activation of its receptors. Furthermore, the data suggest that SRIF influences RPE cell proliferation in a tyrosine-phosphatase dependent manner.

Keywords: retinal pigment epithelium • proliferation • apoptosis/cell death 
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