Purchase this article with an account.
V. Enzmann, L. M. Franco, Y. Katagari, B. W. Row, H. J. Kaplan, P. DeMarco, M. A. McCall, S. Wolf; Sodium Iodate-Generated Retinal Pigment Epithelium Dystrophy - A Model of Induced Retinal Cell Damage. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2530.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To characterize morphological and functional changes in the mouse eye after systemic administration of an intermediate sodium iodate dose over an extended time period.
Damage to the retinal pigment epithelium (RPE) was generated with a single intravenous injection of 1% sterile sodium iodate (NaIO3), at a dose of 35 mg/kg, in 4-6 week old C57BL/6 mice. Visual function was assessed with both a cued water maze test and a photopic/scotopic electroretinogram (ERG) at 1, 3, 7, 14, 21, and 28 days post-injection (PI). Additionally, RPE autofluorescence in whole eye flat mounts was quantified and H&E staining of retinal sections performed to assess the changes in outer neurosensory retinal anatomy.
A decline in the photopic ERG response was evident as early as day 7 PI, and both scotopic and photopic ERG responses showed a progressive decline until no scotopic response was obtained on day 28 PI. However, visual function, as measured by the cued water maze test, was not significantly decreased until day 14 PI. A significant decrease in the thickness of the outer segments and outer nuclear layer, as well as the number of photoreceptor nuclei, was observed as early as day 7 PI. In contrast, no alteration in the outer plexiform layer was observed. Autofluorescence of the RPE decreased by 315% on day 28 PI compared to controls.
We observed a direct correlation between anatomical cell loss in the photoreceptor layer of the neurosensory retina and decreased behavioral and electrophysiologic function after systemic sodium iodate injection in the mouse. These changes progressed over time. The NaIO3 model of retinal damage can be manipulated and used in studies of the efficacy of cellular therapeutics and transplantation.
This PDF is available to Subscribers Only