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M. Fujihara, K. Yamashiro, Y. Kurimoto, M. Takahashi, T. Asahara, J. T. Handa; A New Method to Detect Retinal Vascular Leakage With HRA2. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2579.
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© ARVO (1962-2015); The Authors (2016-present)
Retinal edema can result in severe visual loss. An easy method to detect retinal vascular leakage in an animal model of retinal edema would be indispensable for investigation of new treatment strategy. The purpose of the current study was to establish a new method for detecting retinal vascular leakage in endotoxin-induced uveitis (EIU) in a mouse with HRA2.
EIU was induced in C57BL/6J mice (n=51) by intravitreous injection of lipopolysaccharide (LPS). At various time points (0, 24, 48, 72hours) after LPS injection, fluorescein sodium salt solution was injected intraperitoneally. The fundus was observed with HRA2 using fluorescein angiography 24 hours after fluorescein injection.
The retinal vascular leakage could be detected as remnant fluorescence of the retina in all EIU mice and could not be detected in normal. The degree of the leakage could be easily classified from stage 0 to stage 4 and these correlate with the injected volume of LPS.
We successfully established a novel method to detect retinal vascular leakage in EIU mice. Our new method would be a great help to develop new treatment strategies for retinal edema.
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