May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Quantitative Fluorescein Angiograms
Author Affiliations & Notes
  • A. Rosa
    Coimbra University Hospital, Center of Ophthalmology, Institute of Biomedical Research on Light and Image, Coimbra, Portugal
  • P. Baptista
    AIBILI–Association for Biomedical Research and Innovation on Light and Image, Coimbra, Portugal
  • A. Santos
    AIBILI–Association for Biomedical Research and Innovation on Light and Image, Coimbra, Portugal
  • M. Soares
    Coimbra University Hospital, Center of Ophthalmology, Institute of Biomedical Research on Light and Image, Coimbra, Portugal
  • R. Bernardes
    AIBILI–Association for Biomedical Research and Innovation on Light and Image, Coimbra, Portugal
  • A. Schwartz
    Center for Quantitative Cytometry, San Juan, Puerto Rico
  • W. Carter
    Pfizer, Inc., Groton, Connecticut
  • J. Cunha-Vaz
    AIBILI–Association for Biomedical Research and Innovation on Light and Image, Coimbra, Portugal
    Faculty of Medicine, University of Coimbra, Coimbra University Hospital, Center of Ophthalmology, Institute of Biomedical Research on Light and Image, Coimbra, Portugal
  • Footnotes
    Commercial Relationships A. Rosa, None; P. Baptista, None; A. Santos, None; M. Soares, None; R. Bernardes, Inventor on patent application, P; A. Schwartz, Consultant for Pfizer, Inc., C; Inventor designated on a patent application on presentation material, P; W. Carter, Company funding the research, E; J. Cunha-Vaz, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2587. doi:
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    • Get Citation

      A. Rosa, P. Baptista, A. Santos, M. Soares, R. Bernardes, A. Schwartz, W. Carter, J. Cunha-Vaz; Quantitative Fluorescein Angiograms. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2587.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Clinical assessment of quantitative fluorescein angiograms based on novel instrumentation adaptation.

Methods:: Fluorescein angiograms were simultaneously performed on patient’s retinas and on standard cells composed of two sets of five standard fluorescein solutions mimicking the environmental conditions of the plasma and vitreous, respectively. From automatically established calibration plots (on a scan basis), regular gray-scale fluorescein angiograms were converted into absolute concentration image maps independent from instrumentation setup and operator. Diabetic retinopathy eyes, eyes before and 3 days after laser photocoagulation, and fellow-eyes from patients with choroidal neovascularization were imaged using this new technique.

Results:: Repetitive measurements over patients clearly show the advantage of correcting for instrumentation setup. Comparison of scans taken before and after laser photocoagulation demonstrates background values within the same range (serum[ng/ml]/vitreous [ng/ml]), 329/197 to 366/191 and the expected increase over laser spots of 869/352 to 2479/761, 1611/510 to 2504/766 and 435/232 to 2395/742. Background values were found to range from 170/108 to 340/160 for non-photocoagulated eyes.

Conclusions:: Quantitative fluorescein angiograms compare favorably with conventional fluorescein angiograms by removing instrumentation setup bias and by allowing absolute concentration image maps to be built and comparable. Furthermore, the use of a non-linear color-coded map allows the detection of subtle differences not visible on regular fluorescein angiograms. It is realized that this first step needs to be complemented by other standardization procedures, e.g., by fundus reflectance.

Keywords: clinical research methodology • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • imaging/image analysis: clinical 
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