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G. Cappai, C. Malloci, I. Zucca, M. Galantuomo, M. Fossarello; Fundus Autofluorescence Intensity Variation After Dark and Light Adaptation in Normal Subjects. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2594.
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To evaluate in vivo in normal emmetropic subjects with different ages the spatial distribution and kinetics of fundus autofluorescence (AF) in darkness and after exposure to bright bleaching light.
Eight young phakic subjects, 6 males and 2 females (mean age: 32 yrs ± 5.86) and 6 aged pseudophakic subjects, 2 males and 4 females (mean age: 64 yrs ± 6.35) were included in the study. Clinical examination included refraction, uncorrected visual acuity (UVA) at distance using decimal Snellen and ETDRS charts, slit lamp biomicroscopy and funduscopy, fundus photography TOPCON TRC 50X, Topcon Optical, Tokyo, Japan). Pupils were dilated using tropicamide 1% and phenylephrine 10%. The fundus AF imaging was performed with a Heidelberg scanning laser ophthalmoscope (HRA1).For every subject we obtained AF images in steady condition of SLO lighting in the following conditions: after dark adaptation (30 min), after bleaching (5 min), and after a further dark adaptation (30 min).
In young individuals we observed similar AF intensity changes in the different retinal adaptation states; in particular, after dark adaptation there is a considerable reduction of AF intensity; while after bleaching, there is a partial recovery of AF intensity. After the second period of dark adaptation, a further reduction of AF intensity is observed. The differences in autofluorescence relative intensity during the time of dark-light adaptation were statistically significant (P<0.05). In the aged group, all individuals showed profiles of AF changes similar to those observed in the young group, but AF intensity variations resulted significantly lower.
This study emphasizes the dynamic aspect of AF fundus images in vivo.It is supposable that the intrinsic fluorescence of all-trans retinol generates spectral emissions similar to those of lipofuscin.
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