May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
The Role of CXC Chemokine Receptor Two in Pseudomonas aeruginosa and Staphylococcus aureus Corneal Infection
Author Affiliations & Notes
  • N. Cole
    Institute for Eye Research, Kensington, Australia
    Vision CRC, Sydney, Australia
  • E. B. Hume
    Institute for Eye Research, Kensington, Australia
    School of Optometry and Vision Science, University of New South Wales, Sydney, Australia
  • S. Khan
    Vision CRC, Sydney, Australia
    School of Optometry and Vision Science, University of New South Wales, Sydney, Australia
  • L. L. Garthwaite
    School of Optometry and Vision Science, University of New South Wales, Sydney, Australia
  • T. C. R. Conibear
    School of Optometry and Vision Science, University of New South Wales, Sydney, Australia
    Vision CRC, sydney, Australia
  • D. Miles
    Vision CRC, Sydney, Australia
    School of Optometry and Vision Science, University of New South Wales, Sydney, Australia
  • Y. Aliwarga
    Institute for Eye Research, Kensington, Australia
  • M. B. Krockenberger
    Faculty of Veterinary Science, University of Sydney, Sydney, Australia
  • M. D. P. Willcox
    Institute for Eye Research, Kensington, Australia
    Vision CRC, Sydney, Australia
  • Footnotes
    Commercial Relationships N. Cole, None; E.B. Hume, None; S. Khan, None; L.L. Garthwaite, None; T.C.R. Conibear, None; D. Miles, None; Y. Aliwarga, None; M.B. Krockenberger, None; M.D.P. Willcox, None.
  • Footnotes
    Support NHMRC 222826
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2660. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      N. Cole, E. B. Hume, S. Khan, L. L. Garthwaite, T. C. R. Conibear, D. Miles, Y. Aliwarga, M. B. Krockenberger, M. D. P. Willcox; The Role of CXC Chemokine Receptor Two in Pseudomonas aeruginosa and Staphylococcus aureus Corneal Infection. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2660.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: Despite the use of antibiotics for microbial keratitis, the host inflammatory response continues to cause damage to the cornea, which may lead to blindness. CXC receptor 2 binding chemokines have been implicated in the pathogenesis of P. aeruginosa keratitis, however its exact role in P. aeruginosa keratitis and its role in S. aureus keratitis remains to be elucidated.

Methods:: Corneas of CXC receptor 2 knockout and wildtype mice (Cmkar -/- & Cmkar +/+) were scratched and 1 x 108 or and 2 x 106 colony forming units/mL of S. aureus or P.aeruginosa were added to corneas respectively, contralateral eyes served as a scratch control. Twenty-four hours post-infection, mice were sacrificed and eyes harvested for enumeration of bacteria, myeloperoxidase levels (MPO), chemokines and LTB4.

Results:: For S. aureus infected eyes Cmkar -/- had greater than 10 fold more bacteria than WT mice. Levels of chemokines (KC, MIP-2 & MCP-1) were significantly elevated in gko mice (p < 0.05). For P. aeruginosa eyes Cmkar -/- had 20-100 fold more bacteria than WT mice. Levels of chemokines (KC, MIP-2 & LTB4) were significantly elevated in gko mice (p < 0.05). No differences in MPO levels between gko and WT mice for both bacterial strains.

Conclusions:: A lack of CXC receptor 2 leads to an inability to control bacterial numbers in spite of similar levels of infiltrating cells as a result of compensatory expression of chemokines. This may indicate a pivotal role of the CXC2 receptor in neutrophil activation.

Keywords: inflammation • keratitis • cytokines/chemokines 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×