May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Adjuvant Treatment Using Dialyzable Leukocyte Extract for Herpetic Keratitis
Author Affiliations & Notes
  • G. A. Luna-Baca
    Institute of Ophthalmology Conde de Valenciana, Mexico City, Mexico
    Research Unit,
  • M. Linares
    Institute of Ophthalmology Conde de Valenciana, Mexico City, Mexico
    Research Unit,
  • C. Santacruz-Valdes
    Institute of Ophthalmology Conde de Valenciana, Mexico City, Mexico
    Cornea and Refractive Surgery Department,
  • G. Aguilar-Velazquez
    Institute of Ophthalmology Conde de Valenciana, Mexico City, Mexico
    Research Unit,
  • R. Chavez
    Laboratorio de Inmunologia, Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
  • M. Perez-Tapia
    Department of Immunology, National School of Biological Sciences, ENCB-IPN, Mexico City, Mexico
  • I. Estrada-Garcia
    Department of Immunology, National School of Biological Sciences, ENCB-IPN, Mexico City, Mexico
  • S. Estrada-Parra
    Department of Immunology, National School of Biological Sciences, ENCB-IPN, Mexico City, Mexico
  • M. C. Jimenez-Martinez
    Institute of Ophthalmology Conde de Valenciana, Mexico City, Mexico
    Research Unit,
  • Footnotes
    Commercial Relationships G.A. Luna-Baca, None; M. Linares, None; C. Santacruz-Valdes, None; G. Aguilar-Velazquez, None; R. Chavez, None; M. Perez-Tapia, None; I. Estrada-Garcia, None; S. Estrada-Parra, None; M.C. Jimenez-Martinez, None.
  • Footnotes
    Support Fundación Conde de Valenciana and CONACYT SALUD 2005-14-108
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2666. doi:
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    • Get Citation

      G. A. Luna-Baca, M. Linares, C. Santacruz-Valdes, G. Aguilar-Velazquez, R. Chavez, M. Perez-Tapia, I. Estrada-Garcia, S. Estrada-Parra, M. C. Jimenez-Martinez; Adjuvant Treatment Using Dialyzable Leukocyte Extract for Herpetic Keratitis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2666.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Introduction:: The immune response to herpetic keratitis begins on the early stage of viral replication, attracting T lymphocytes to the site of lesion and producing large quantities of IFNγ. It is known that dialyzable leukocyte extract (DLE) has the ability to modulate the immune response, probably due to IFNγ production. Therefore, DLE has been used in the treatment of viral infections like herpes simplex and herpes zoster.

Purpose:: To evaluate the cytokine production profile by peripheral blood mononuclear cells (PBMC) from herpetic keratitis patients (HK-p) treated with dialyzable leukocyte extract.

Methods:: CD4, CD8, IFNγ, IL-4, perforine and granzime expression was determined by flow cytometry on PBMC from HK-p, before (BDLE) and after (ADLE) treatment with DLE and also on PBMC from healthy controls (HC).

Results:: Percentage (%) CD4+IFNγ+ T cells in HK-p BDLE= 15 vs 9 HC (p=0.01) vs 33 ADLE (p=0.02) (p= 0.009 HC vs ADLE); %CD4+ IL-4+ T cells in HK-p BDLE= 20 vs 12 HC vs 6.5 ADLE; %CD8+IFNγ+ T cells in HK-p BDLE= 23 vs 25 HC vs 50 ADLE (p=0.002 BDLE vs ADLE, p=0.008 ADLE vs HC); %CD8+IL-4+ T cells in HK-p BDLE= 7 vs 10 HC vs 10 ADLE; %CD8+IL-4+ T cells= 7 vs 23 CD8+IFNγ+ T cells in HK-p BDLE (p=0.005); %CD8+IL-4+ T cells= 10 vs 50 CD8+IFNγ+ T cells in HK-p ADLE (p=0.003); %CD4+Granzime+ T cells in HK-p BDLE= 9.5 vs 9.4 HC vs 10.7 ADLE; %CD4+Perforine+ T cells in HK-p BDLE= 5.7 vs 1.1 HC vs 9 ADLE; %CD4+Granzime+Perforine+ T cells in HK-p BDLE= 8.1 vs 2.7 HC vs 10.8 ADLE; %CD8+Granzime+ T cells in HK-p BDLE= 12 vs 26 HC (p=0.01) vs 10.7 ADLE (p=0.008 vs HC); %CD8+Perforine+ T cells in HK-p BDLE= 8.5 vs 4.8 HC vs 13.2 ADLE; %CD8+Granzime+Perforine+ T cells in HK-p BDLE= 32.8 vs 33.5 HC vs 37.1 ADLE.

Conclusions:: CD8+ and CD4+ T cells from HK-p predominantly produce IFNγ after treatment with dialyzable leukocyte extract. This increase of cytokine production could explain the better outcomes by a citotoxic T cell response in the studied patients.

Keywords: cornea: clinical science • herpes simplex virus • immunomodulation/immunoregulation 
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