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M. Banitt, D. Ritterband, M. Shah, J. Seedor, R. Koplin; Efficacy a Safety of Moxifloxacin as an Additive in Optisol-GS, a Preservation Medium for Corneal Donor Tissue. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2697.
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To assess the microbiological efficacy and safety of moxifloxacin (500µg/ml) as an additive to Optisol GS® to reduce bacterial corneal donor rim contamination.
342 donor rims were studied. One half of each donor rim was placed in standard Optisol GS® and the other half rim in Optisol GS® fortified with moxifloxacin (500µg/ml). All rims were refrigerated for 24 hours at 3º C and placed in thioglycolate broth and incubated at 37º C for 7 days. One pair of donor buttons not used in transplantation stored in each solution was examined for endothelial changes using electron microscopy (EM). A second pair of cornea buttons was examined for toxicity by endothelial staining with 0.3% trypan blue and 0.2% alizarin red.
The percentage of culture positive donor rims in the Optisol GS® group was 6.1% (21/342) and in the moxifloxacin-fortified Optisol GS® media was 0.6% (2/342). The difference was statistically significant (p ≤ 0.001; Chi-square test). Nineteen bacteria grew in standard Optisol GS® media (S. epidermidis 10, P.acnes 8, S. aureus 1). No bacteria grew in the moxifloxacin-fortified Optisol GS®. Two cultured rims grew C. albicans in both media. No difference in toxicity was found on EM or in the bioassay.
The addition of 500 µg/ml of moxifloxacin significantly reduces the rate of bacterial growth in corneal rims stored in Optisol GS® without causing endothelial toxicity.
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