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L. L. Camit, F. Lamoke, A. Choi, H. Lim, X. Yang, P. A. Wood, W. J. M. Hrushesky, W. J. Jahng; Circadian Clock Controls RPE65 in Melatonin Deficient Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2957.
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Recent studies have shown that RPE65 and lecithin retinol acyltransferase (LRAT) controls the biosynthesis and fluxes of retinoids in the visual cycle. In order to link the visual cycle with an endogenous circadian rhythm, the visual cycle protein RPE65 was examined whether specific visual cycle protein is under the circadian clock control.
B6.129S7-Per2tm1Brd/J (B6) mice were kept on a twelve hour light-dark cycle or in constant darkness, and whole eyes were harvested every four hours. Whole eye extracts were used in a SDS-PAGE and western blot analysis to determine protein expression. 2-D electrophoresis followed by mass spectrometery was performed in urea buffer to confirm the identity of the target protein.
Western blots have shown differing RPE65 concentrations at subsequent time points in B6 whole eye extraction samples.
It is shown here that circadian clock regulates RPE65 expression as one of the control elements of the visual cycle.
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