May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Circadian Clock Controls RPE65 in Melatonin Deficient Mice
Author Affiliations & Notes
  • L. L. Camit
    University of South Carolina School of Medicine, Colulmbia, South Carolina
    Ophthalmology,
  • F. Lamoke
    University of South Carolina School of Medicine, Colulmbia, South Carolina
    Ophthalmology,
  • A. Choi
    University of South Carolina School of Medicine, Colulmbia, South Carolina
    Ophthalmology,
  • H. Lim
    University of South Carolina School of Medicine, Colulmbia, South Carolina
    Ophthalmology,
  • X. Yang
    The Center for Colon Cancer, Dorn Veterans' Affairs Medicial Center, Colulmbia, South Carolina
  • P. A. Wood
    The Center for Colon Cancer, Dorn Veterans' Affairs Medicial Center, Colulmbia, South Carolina
  • W. J. M. Hrushesky
    The Center for Colon Cancer, Dorn Veterans' Affairs Medicial Center, Colulmbia, South Carolina
  • W. J. Jahng
    University of South Carolina School of Medicine, Colulmbia, South Carolina
    Ophthalmology/ Pathology, Microbiology and Immunology,
  • Footnotes
    Commercial Relationships L.L. Camit, None; F. Lamoke, None; A. Choi, None; H. Lim, None; X. Yang, None; P.A. Wood, None; W.J.M. Hrushesky, None; W.J. Jahng, None.
  • Footnotes
    Support Centenary Program, University of South Carolina, Department of Ophthalmology, University of South Carolina
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2957. doi:
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    • Get Citation

      L. L. Camit, F. Lamoke, A. Choi, H. Lim, X. Yang, P. A. Wood, W. J. M. Hrushesky, W. J. Jahng; Circadian Clock Controls RPE65 in Melatonin Deficient Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2957.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Recent studies have shown that RPE65 and lecithin retinol acyltransferase (LRAT) controls the biosynthesis and fluxes of retinoids in the visual cycle. In order to link the visual cycle with an endogenous circadian rhythm, the visual cycle protein RPE65 was examined whether specific visual cycle protein is under the circadian clock control.

Methods:: B6.129S7-Per2tm1Brd/J (B6) mice were kept on a twelve hour light-dark cycle or in constant darkness, and whole eyes were harvested every four hours. Whole eye extracts were used in a SDS-PAGE and western blot analysis to determine protein expression. 2-D electrophoresis followed by mass spectrometery was performed in urea buffer to confirm the identity of the target protein.

Results:: Western blots have shown differing RPE65 concentrations at subsequent time points in B6 whole eye extraction samples.

Conclusions:: It is shown here that circadian clock regulates RPE65 expression as one of the control elements of the visual cycle.

Keywords: retinal pigment epithelium • circadian rhythms • proteomics 
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