May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Real-Time in vivo Imaging of Retinal Apoptosis After Argon Laser Exposure
Author Affiliations & Notes
  • F. W. Fitzke
    UCL Institute of Ophthalmology, London, United Kingdom
    Visual Sciences,
  • S. Schmitz-Valckenberg
    UCL Institute of Ophthalmology, London, United Kingdom
    Visual Sciences,
    Glaucoma & Retinal Neurodegeneration Research Group,
  • L. Guo
    UCL Institute of Ophthalmology, London, United Kingdom
    Glaucoma & Retinal Neurodegeneration Research Group,
  • A. Maass
    UCL Institute of Ophthalmology, London, United Kingdom
    Glaucoma & Retinal Neurodegeneration Research Group,
  • W. Cheung
    UCL Institute of Ophthalmology, London, United Kingdom
    Glaucoma & Retinal Neurodegeneration Research Group,
  • S. E. Moss
    UCL Institute of Ophthalmology, London, United Kingdom
    Cell Biology,
  • P. Munro
    UCL Institute of Ophthalmology, London, United Kingdom
    Clinical Ophthalmology,
  • M. F. Cordeiro
    UCL Institute of Ophthalmology, London, United Kingdom
    Glaucoma & Retinal Neurodegeneration Research Group,
    Western Eye Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships F.W. Fitzke, None; S. Schmitz-Valckenberg, None; L. Guo, None; A. Maass, None; W. Cheung, None; S.E. Moss, None; P. Munro, None; M.F. Cordeiro, None.
  • Footnotes
    Support Wellcome Trust
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3041. doi:
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    • Get Citation

      F. W. Fitzke, S. Schmitz-Valckenberg, L. Guo, A. Maass, W. Cheung, S. E. Moss, P. Munro, M. F. Cordeiro; Real-Time in vivo Imaging of Retinal Apoptosis After Argon Laser Exposure. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3041.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Development of apoptosis in laser photocoagulation has not been previously studied. Using the recently established technique of DARC (detection of apoptosing retinal cells), we investigate for the first time, as far as we are aware, the occurence of in vivo retinal apoptosis following argon laser exposure.

Methods:: Fluorescent labelled annexin 5 was injected into the vitreous of 8 anaesthetised dark agouti rats and argon laser lesions were placed on the retina in both eyes (up to 500 mW, 500 ms). Retinal images were recorded in vivo with DARC technology at baseline and up to one hour after laser application using a confocal scanning laser ophthalmoscope (cSLO). Subsequently, eyes were analysed histologically with confocal scanning microscopy.

Results:: Enlargement and swelling of the retinal laser lesions immediately after treatment was observed on in vivo images. Within 20 minutes, hyperfluorescent spots corresponding to apoptosing cells were identified inside the lasered lesions which appeared to increase over time. Using 3D tomography, these apoptosing spots were located in the inner retinal layers. This was confirmed by histology and cross sectional analysis.

Conclusions:: DARC has previously been used to delineate retinal ganglion cell changes. This is the first time this technique has been use to determine effects on other retinal cells. The ability to monitor changes as they occur and longitudinally, promises to be a major advance in the real time assessment of retinal diseases.

Keywords: apoptosis/cell death • imaging/image analysis: non-clinical • laser 
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