May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Omega-3 Long-Chain Polyunsaturated Fatty Acids (LCPUFAs) Suppress Retinal COX-2 mRNA and Retinal Neovascularization
Author Affiliations & Notes
  • A. Higuchi
    Ophthalmology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts
  • K. M. Connor
    Ophthalmology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts
  • C. M. Aderman
    Ophthalmology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts
  • J. Chen
    Ophthalmology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts
  • J. P. SanGiovanni
    Division of Epidemiology and Clinical Research, National Eye Institute/NIH, Bethesda, Maryland
  • N. N. Salem, Jr.
    Laboratory of Membrane Biochemistry and Biophysics, NIH/National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland
  • E. Y. Chew
    Division of Epidemiology and Clinical Research, National Eye Institute/NIH, Bethesda, Maryland
  • S. Majchrzak
    Laboratory of Membrane Biochemistry and Biophysics, NIH/National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland
  • L. E. H. Smith
    Ophthalmology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts
  • Footnotes
    Commercial Relationships A. Higuchi, None; K.M. Connor, None; C.M. Aderman, None; J. Chen, None; J.P. SanGiovanni, None; N.N. Salem, None; E.Y. Chew, None; S. Majchrzak, None; L.E.H. Smith, None.
  • Footnotes
    Support The V. Kann Rasmussen Foundation and NIH (EY008670 HIGHWIRE EXLINK_ID="48:5:3416:1" VALUE="EY008670" TYPEGUESS="GEN" /HIGHWIRE , EY017017 HIGHWIRE EXLINK_ID="48:5:3416:2" VALUE="EY017017" TYPEGUESS="GEN" /HIGHWIRE EY14811 (LEHS); 5 T32 EY07145 (KMC) P50-DE016191 HIGHWIRE EXLINK_ID="48:5:3416:3" VALUE="DE016191" TYPEGUESS="GEN" /HIGHWIRE , Children's Hospital Boston MRDDRC P01 HD18655, Lew Wasserman Merit Award (LEHS)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3416. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. Higuchi, K. M. Connor, C. M. Aderman, J. Chen, J. P. SanGiovanni, N. N. Salem, Jr., E. Y. Chew, S. Majchrzak, L. E. H. Smith; Omega-3 Long-Chain Polyunsaturated Fatty Acids (LCPUFAs) Suppress Retinal COX-2 mRNA and Retinal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3416.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: Lipid based molecules act as effectors of angiogenesis and inflammation; among the most potent are the eicosanoids, derived from the 20 carbon long chain polyunsaturated fatty acids (LCPUFAs), through the cyclooxygenase (COX) and lipoxygenase (LOX) pathways. Retinal expression of COX and LOX is both constitutive and inducible. COX-2 inhibition is protective against retinopathy. Omega-3 LCPUFAs exhibit anti-inflammatory properties. We have found that dietary intake of ω-3 LCPUFAs suppresses proliferative retinopathy in a mouse model of oxygen-induced retinopathy (OIR). Here we demonstrate that ω-3 LCPUFAs may inhibit pathologic neovascularization through suppression of COX-2 mRNA in this same model.

Methods:: C57Bl/6 mothers were fed (from postnatal day 0 (P0)) a diet enriched with ω-3 LCPUFA or a diet elevated in ω-6 LCPUFA. LCPUFA composition of the mother’s diet was reflected in the milk. The change in ω-6/ω-3 ratio was also reflected in retina of the pups. To induce retinopathy the mothers and pups were placed in 75% oxygen for 5 days from postnatal day 7 (P7) to P12 and then returned to room air. Retinas were isolated at P8 and P14. Retinas were pooled across dietary groups, RNA was isolated, and converted to cDNA. Retinal COX-2 mRNA expression was compared to cyclophilin and quantified using real time-PCR.

Results:: An ω-3 LCPUFA diet versus an ω-6 LCPUFA diet was associated with a 40% inhibition of retinal neovascularization in the OIR mouse model at P17. In ω-3 fed versus ω-6 fed mice in the OIR model there was a 10 fold reduction of COX-2 mRNA at P8 and a 5 fold reduction of COX-2 mRNA at P14. Thus retinal COX-2 mRNA expression with ω-3 LCPUFA intake was significantly suppressed at P8 and P14 when compared to ω-6 LCPUFA intake.

Conclusions:: This finding suggests that an ω-3 LCPUFA diet may inhibit retinal neovascularization in part through altering COX-2 mRNA levels.

Keywords: neovascularization • lipids • inflammation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×