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S. Chattopadhyay, J. O'Rourke, R. E. Cone; Influence of CD94/NKG2 Receptors for Qa-1 on the Suppression of Delayed-Type Hypersensitivity Induced by the Intracameral Injection of Antigen. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3630.
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The presence of antigen in the anterior chamber (AC) induces the systemic suppression of cell-mediated immunity to the antigen that is effected by splenic CD8+ suppressor-effector T cells. Activation of these CD8+ T cells is dependent on Qa-1 antigens on B cells that may present antigen received from F4/80+ cells. In addition, the suppression of Delayed-Type hypersensitivity (DTH) by the suppressor-effector cells is associated with recipient Qa-1 antigens. Here we investigate the role of CD94/NKG2 receptors for Qa-1 in the suppression of DTH induced by Anterior Chamber-Associated Immune Deviation (ACAID).
Suppression of DTH to ovalbumin (OVA) and the production of splenic suppressor-effector cells were investigated in BALB/c, C57Bl/6, DBA/2NCr mice and CD94-deficient DBA/2J mice that had received intracameral OVA. Suppressor-effector spleen cells were recovered from AC-injected, OVA-immunized mice (AC-SPL cells) one week following immunization. AC-SPL cell suppressive activity was measured in a modified local adoptive transfer system by injection of AC-SPL cells + OVA into the footpads of OVA-immunized mice.
Neither DTH suppression nor suppressor-effector AC- SPL cells were induced in CD94-deficient DBA/2J mice compared to DBA2/NCr, BALB/c and C57Bl/6 control mice. CD94/NKG2, and CD103 expression was decreased in DBA/2J CD8+ T cells. Expression of Qa-1b was reduced in splenic CD19+ B cells and F4/80+ cells in DBA/2J mice, but not DBA/2NCr mice. Admixture of anti-CD94 monoclonal antibody with BALB/c AC- SPL cells prevented the suppression of DTH by the AC-SPL cells.
These observations suggest a role for both CD94/NKG2 receptors and Qa-1 in the suppression of peripheral cell-mediated immunity induced by the injection of antigen into the anterior chamber.
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