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W.-B. Liu, Y. Li, L. Zhang, H.-G. Cheng, J.-P. Liu, D. W.-C. Li; Differential Expression of the Protein Serine/Threonine Phosphatase-1 and -2A in Different Ocular Tissues From Gold Fish and Mouse. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3802.
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Protein serine/threonine phosphatases play important roles in regulating gene expression, cell proliferation, cell differentiation, and apoptosis. We have previously examined the expression patterns of the catalytic subunits for both PP-1 (PP-1c) and PP-2A (PP-2Ac) in bovine and rat lenses. In the present study, we have extended our observations on the relative expression levels of these two phosphatases in retina, lens and cornea of another two species of vertebrates, goldfish and mouse.
Mice of 4 weeks in age and adult gold fish of 6 months were used for this study. The eyeballs were first dissected and different ocular tissues were dissected out on ice. The dissected tissues were frozen with liquid nitrogen and then used for RNA and protein extractions. RT-PCR was used to detect the mRNA expression patterns. Western blot was used to analyzed the protein expression levels.
Our results demonstrated several important features for the expression patterns of the catalytic subunits for PP-1 (PP-1c) and PP-2A (PP-2Ac): 1) the expression level of PP-1c is much stronger than that for PP-2Ac in all the ocular tissues of either goldfish or mouse; 2) both PP-1c and PP-2Ac have the highest levels of expression in retina of both organisms; 3) in the ocular lenses, the expression levels of both PP-1c and PP-2Ac are higher in the epithelial cells than in the fiber cells; 4) in cornea, the expression levels of both PP-1c and PP-2Ac are much lower in goldfish than that in mouse.
PP-1 is a more abundant phosphatase than PP-2A in both goldfish and mouse eyes, and that the genes encoding PP-1c and PP-2Ac are differentially expressed in the ocular tissues from both mouse and gold fish.
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