May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Differential Expression of the Protein Serine/Threonine Phosphatase-1 and -2A in Different Ocular Tissues From Gold Fish and Mouse
Author Affiliations & Notes
  • W.-B. Liu
    College of Life Sciences, Hunan Normal University, Changsha Hunan, China
  • Y. Li
    College of Life Sciences, Hunan Normal University, Changsha Hunan, China
  • L. Zhang
    College of Life Sciences, Hunan Normal University, Changsha Hunan, China
  • H.-G. Cheng
    College of Life Sciences, Hunan Normal University, Changsha Hunan, China
    Department of Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • J.-P. Liu
    Department of Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • D. W.-C. Li
    College of Life Sciences, Hunan Normal University, Changsha Hunan, China
    Department of Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships W. Liu, None; Y. Li, None; L. Zhang, None; H. Cheng, None; J. Liu, None; D.W. Li, None.
  • Footnotes
    Support Supported by 1R01 EY15765, University of Nebraska Medical Center and the Lotus Scholar Professorship Funds.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3802. doi:
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      W.-B. Liu, Y. Li, L. Zhang, H.-G. Cheng, J.-P. Liu, D. W.-C. Li; Differential Expression of the Protein Serine/Threonine Phosphatase-1 and -2A in Different Ocular Tissues From Gold Fish and Mouse. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3802.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Protein serine/threonine phosphatases play important roles in regulating gene expression, cell proliferation, cell differentiation, and apoptosis. We have previously examined the expression patterns of the catalytic subunits for both PP-1 (PP-1c) and PP-2A (PP-2Ac) in bovine and rat lenses. In the present study, we have extended our observations on the relative expression levels of these two phosphatases in retina, lens and cornea of another two species of vertebrates, goldfish and mouse.

Methods:: Mice of 4 weeks in age and adult gold fish of 6 months were used for this study. The eyeballs were first dissected and different ocular tissues were dissected out on ice. The dissected tissues were frozen with liquid nitrogen and then used for RNA and protein extractions. RT-PCR was used to detect the mRNA expression patterns. Western blot was used to analyzed the protein expression levels.

Results:: Our results demonstrated several important features for the expression patterns of the catalytic subunits for PP-1 (PP-1c) and PP-2A (PP-2Ac): 1) the expression level of PP-1c is much stronger than that for PP-2Ac in all the ocular tissues of either goldfish or mouse; 2) both PP-1c and PP-2Ac have the highest levels of expression in retina of both organisms; 3) in the ocular lenses, the expression levels of both PP-1c and PP-2Ac are higher in the epithelial cells than in the fiber cells; 4) in cornea, the expression levels of both PP-1c and PP-2Ac are much lower in goldfish than that in mouse.

Conclusions:: PP-1 is a more abundant phosphatase than PP-2A in both goldfish and mouse eyes, and that the genes encoding PP-1c and PP-2Ac are differentially expressed in the ocular tissues from both mouse and gold fish.

Keywords: gene/expression • signal transduction • phosphorylation 
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