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A. Schnaidt, A. Osvald, F. Schirra, L. Troeber, U. Löw, C. Jonescu-Cuypers, B. Seitz; Comparison of the Measurement of Corneal Endothelial Cell Density (ECD) by Two Different Non-Contact Specular Microscopes. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3870.
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The purpose of the study was to evaluate differences between endothelial cell counts by means of the semi-automatic SP-2000P Specular Microscope (SM) (Topcon/Japan) and the novel video-based Sea Eagle System (SES) (Rhine-Tec/Germany). Determination of endothelial cell density is crucial for diagnosis in corneal disorders, especially before and after corneal surgery (e.g. keratoplasty).
Measurements were performed on 100 corneas of 50 patients with corneal diseases. An image was acquired with both devices and the number of cells was subsequently calculated. The SM performs a semi-automatic cell count measurement, whereas the SES cell detection runs automatically. Measurement data was compared and statistically evaluated.
Mean age of patients was 59.8 years (SD 11.2). Mean cell count for the right eyes was 2192.9 cells/mm² (SD 576.5) for SM and 2163.3 cells/mm² (SD 534.0) for SES respectively. Left eyes` cell count was 2340.6 cells/mm² (SD 519.6) for SM and 2316.6 cells/mm² (SD 474.5) for SES respectively. No statistically significant difference was found between both devices (p=0.32 and p=0.3). Pearson's correlation coefficient was 0.843 for the right eyes and 0.901 for the left eyes, respectively.
Both specular microscopic devices for endothelial cell count are comparable for clinical use in terms of precision. However, with the SES direct on-screen comparison with previous measurements is possible and therefore more applicable for routine follow-up.
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