May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Effects of Cyclic Intraocular Pressure Oscillations on Tissue Viability of Human Anterior Segments in Organ Culture
Author Affiliations & Notes
  • R. F. Ramos
    Ophthalmology, University of Arizona, Tucson, Arizona
  • W. D. Stamer
    Ophthalmology, University of Arizona, Tucson, Arizona
  • Footnotes
    Commercial Relationships R.F. Ramos, None; W.D. Stamer, None.
  • Footnotes
    Support EY016887 (RFR), EY17007 (WDS), RPB Foundation (WDS)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 3928. doi:
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      R. F. Ramos, W. D. Stamer; Effects of Cyclic Intraocular Pressure Oscillations on Tissue Viability of Human Anterior Segments in Organ Culture. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3928.

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Abstract

Purpose:: Cyclic oscillations in intraocular pressure (IOP) of human eyes occur in association with each heartbeat, averaging 2.7 mmHg. Using a modified version of the anterior segment perfusion system, we modeled IOP oscillations and observed a concurrent decrease in outflow facility (-27.6 ± 18%, n=7). The aim of the present study was to determine the impact of intraocular pulse on viability of ocular tissues in organ culture.

Methods:: Using an anterior segment perfusion model, post-mortem human eyes were perfused at a constant rate until reaching a stable baseline within a physiological range (0.15-0.40 µl/min/mmHg). Intraocular pulsations were then introduced to the system using two pumps in tandem, a syringe pump and a pulsatile blood pump, to simulate the magnitude and frequency of intraocular pressure oscillations found in vivo. Central corneal thickness (CCT) was monitored over time of perfusion as a real time viability indicator. Saggital sections through conventional outflow tissues in four quadrants of each perfused segment were processed by standard histology and evaluated in a masked fashion using a standard scoring system.

Results:: Anterior segments exposed to intraocular pressure oscillations, and their paired controls were not significantly different in terms cellularity of conventional outflow tissues. Both real time (CCT) and post-perfusion (histological scoring) methods of viability evaluation show no significant differences between segments exposed to pressure oscillations and their paired controls (p=0.725).

Conclusions:: Results suggest that the decrease in outflow facility observed in response to cyclic intraocular pressure oscillations is not a function of damage to cells or structures in the conventional outflow pathway, but rather an active cellular response to the mechanical stimulus.

Keywords: intraocular pressure • anterior segment • outflow: trabecular meshwork 
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