Purchase this article with an account.
R. F. Ramos, W. D. Stamer; Effects of Cyclic Intraocular Pressure Oscillations on Tissue Viability of Human Anterior Segments in Organ Culture. Invest. Ophthalmol. Vis. Sci. 2007;48(13):3928.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Cyclic oscillations in intraocular pressure (IOP) of human eyes occur in association with each heartbeat, averaging 2.7 mmHg. Using a modified version of the anterior segment perfusion system, we modeled IOP oscillations and observed a concurrent decrease in outflow facility (-27.6 ± 18%, n=7). The aim of the present study was to determine the impact of intraocular pulse on viability of ocular tissues in organ culture.
Using an anterior segment perfusion model, post-mortem human eyes were perfused at a constant rate until reaching a stable baseline within a physiological range (0.15-0.40 µl/min/mmHg). Intraocular pulsations were then introduced to the system using two pumps in tandem, a syringe pump and a pulsatile blood pump, to simulate the magnitude and frequency of intraocular pressure oscillations found in vivo. Central corneal thickness (CCT) was monitored over time of perfusion as a real time viability indicator. Saggital sections through conventional outflow tissues in four quadrants of each perfused segment were processed by standard histology and evaluated in a masked fashion using a standard scoring system.
Anterior segments exposed to intraocular pressure oscillations, and their paired controls were not significantly different in terms cellularity of conventional outflow tissues. Both real time (CCT) and post-perfusion (histological scoring) methods of viability evaluation show no significant differences between segments exposed to pressure oscillations and their paired controls (p=0.725).
Results suggest that the decrease in outflow facility observed in response to cyclic intraocular pressure oscillations is not a function of damage to cells or structures in the conventional outflow pathway, but rather an active cellular response to the mechanical stimulus.
This PDF is available to Subscribers Only