May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Vitreous Humor Enhances the Proliferation of Retinal Precursor Cells
Author Affiliations & Notes
  • J. Yang
    Ophthalmology Department, University of California, Irvine, Orange, California
    Department of Medical Anatomy, University of Copenhagen, Copenhagen, Denmark
  • H. Klassen
    Ophthalmology Department, University of California, Irvine, Orange, California
  • M. Pries
    Department of Medical Anatomy, University of Copenhagen, Copenhagen, Denmark
  • W. Wang
    Ophthalmology Department, Peking University Third Hospital, Beijing, China
  • M. H. Nissen
    Department of Medical Anatomy, University of Copenhagen, Copenhagen, Denmark
  • Footnotes
    Commercial Relationships J. Yang, None; H. Klassen, None; M. Pries, None; W. Wang, None; M.H. Nissen, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 4075. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. Yang, H. Klassen, M. Pries, W. Wang, M. H. Nissen; Vitreous Humor Enhances the Proliferation of Retinal Precursor Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):4075.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose:: Intravitreal injection is an important delivery route for studies involving the transplantation of various types of precursor cells to the retina, however, the effect on these cells of exposure to the vitreous microenvironment has not been specifically investigated. Here vitreous humor was evaluated for the potential to stimulate the proliferation of rat retinal precursor cells (RPCs) in vitro.

Methods:: Rat RPCs were isolated at embryonic day 19 and cultured in DMEM:F12 medium containing N2 supplement and 20ng/ml EGF in the presence or absence of fluid expressed from porcine vitreous humor. Cellular proliferation at different concentrations of vitreous fluid supplementation was quantified from day 0 to day 4 using a 3H-thymidine incorporation assay. Active components of vitreous fluid were partially characterized by gel filtration chromatography (GFC) and UV spectral analysis. The effect of each vitreous fraction on RPC proliferation was then determined as well.

Results:: Addition of 20% vitreous fluid to primary rat RPC cultures increased 3H-thymidine incorporation by as much as 481%, as compared to growth medium without vitreous supplementation. One of the vitreous fractions showing growth promoting activity was localized to the low molecular weight range < 1000 Da, consistent with ascorbic acid. The presence of ascorbic acid in the vitreous fluid was confirmed by UV spectral analysis.

Conclusions:: These results show that vitreous fluid enhances the proliferation of rat RPCs in vitro. Ascorbic acid likely contributes to this effect in that we have previously shown this molecule to be pro-proliferative for this cell type. Because vitreous-associated enhancement of RPC proliferation remains a growth-factor-dependent phenomenon, the proliferative status of transplanted cells in the vitreous cavity is likely determined by a combination of these and other factors.

Keywords: regeneration • retinal culture • proliferation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×